Olympus IX81 Brochure & Specs - Page 14

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FRET

Bright, simultaneous two-wavelength imaging using the primary image.

FRET Split imaging system

• Simultaneous two-color split imaging with one CCD camera.

• Unique design splits the primary image for the highest efficiency

and light transmission necessary for weak fluorescence signals

such as CFP/YFP FRET experiments.

•Compact and space-saving design takes advantage of the 70 mm

of free space between the microscope frame and the primary

image plane found on all Olympus Research Upright and Inverted

Microscopes.

• Simple cassette mechanism makes it easy to switch between

split and full frame imaging.

• Unit is up to 10% brighter than similar relay lens based, image

splitting systems.

• When used with the rectangular field stop U-RFSS, excitation

energy is limited to the camera's field of view, minimizing specimen

photo-bleaching.

Rectangular field

stop/U-RFSS

Cube cassette for

full image

Cube cassette for

Split primary image

split images

camera port/U-SIP

YFP

CFP

HeLa cell, in which YC3.1 (cytoplasm) and YC3.1nu (with nuclear localization signal) are coexpressed.

FRET changes are observed through histamine stimulation, and images are acquired at intervals of

50 msec.

Rectangular field stop

Specimen

U-RFSS

Objective

L shape fluorescence illuminator

Fluorescence mirror unit

IX2-RFAL

Filter set such as

XF88-2(OMEGA) or

31044v2(CHROMA)

Split primary image

Filter sliders

Built-in separation

camera port

(Emission, ND sliders)

dichromatic mirror

U-SIP

(DM505)

Excitation filter

Tube lens

Prism

High resolution camera

Research inverted

system microscope

IX81/IX71

Cube cassette for

YFP

CFP

split image

Split image

Mirrors

U-SIP main specifications

Microscope

IX71/81

Image separation

Right and left 2-separation

(can be adjusted independently)

Built-in separation dichromatic mirror

DM505 (special size)

Filter slider

Emission, ND filters' size ø25 mm,

total thickness: 8 mm

Used together with commercially available filter set

(XF88-2 OMEGA) or 31044v2(CHROMA)

Field Number

Split image: 8

Full image: 11

Magnifications

1X (primary image)

Objectives

40X and higher

Camera mounting

C-mount

Recommended camera

Chip size 2/3 inch

* Not available in some areas

Photoactivation

Photoactivation illuminator for inverted microscopy.

Photoactivation Fluorescence

Microscope system

The photoactivation illuminator allows the exposure by UV light to

specific regions of a cell for photoconversion, the uncaging of

compounds and the photoactivation of specific fluorochromes.

• A specified area of the cell can be exposed to UV light while

observing the targeted cell by fluorescence or transmitted (DIC)

method.

• Compliance with FRAP or FLIP

experiments (by special order).

• Easy system upgrade by

attaching double lamphouse

illuminator IX2-RFAW to IX2

series inverted microscope.

Double lamphouse illuminator IX2-RFAW

Setting up example for Kaede

Magnetic shutter

Double lamp

Sample

house illuminator

IX2-RFAW

Excitation filter

BP330-385

Filter slider

Pinhole slider

Field

stop

position *1

Pinhole or slit

Composed dichromatic

Filter slider

mirror of right and left path

Inverted microscope

IX series

Filter wheel 1

such as Lambda10-2

Excitation filter

475AF20(XF1072 OMEGA) or

HQ475/20x(CHROMA)

Excitation filter *2

550DF30(XF1021 OMEGA)

75W xenon apo

lamp housing

U-LH75XEAPO

Power supply unit

The novel Kaede gene is useful in biology because it exhibits photoconversion. Normally, the Kaede

gene shows green fluorescence but after exposure to UV light will exhibit red fluorescence. By using

UV light to only a specific region within a labeled cell and then noting the movement of red beyond

that region, observations of internal cellular dynamics can easily be made. The photo on the left

shows a nerve cell (from a rat hippocampus) pre-labeled with green Kaede gene.

The photo on the right side was taken after the right-most cell body was exposed to a 10 µm

diameter spot of UV light for 60 seconds, thus changing the Kaede gene from green to red. Note the

translocation of the red shifted gene outside of the 10 µm spot thus indicating intracellular transport

mechanisms.

Objective

IX2-RFAW specifications

UPLFLN40XO

Microscope

Fluorescence mirror unit

Pinhole slider

U-MF2+dichromatic mirror

(DM400 on the illustration)

Exposed area on

Fluorescence filter *2

the specimen

575ALP (XF3089 OMEGA) or

Filter slider

HQ575LP(CHROMA)

Excitation filter slider

Fluorescence filter

530DF30(XF3107 OMEGA) or

Filter size

D530/30x(CHROMA)

High resolution

Composed dichromatic mirror

camera

of right and left light path

Power consumption

Dimensions

Filter wheel 2

such as Lambda10-2

*1 Field stop position is the same

position as the focus point of the

sample.

*2 Use 550DF30 excitation filter in the

filter wheel 1 and 575ALP

fluorescence filter in the filter wheel 2

when observing red Kaede protein.

Exchange of the fluorescence mirror

unit is not required.

Application System

IX81/71/51, IX70/50

2-step exchange (pinhole or slit/vacant hole)

Pinhole and slit are available on the market

(ø16 mm Melles Griot Inc. products)

Pinhole diameter

objective magnification

3-step exchange (shutter/filter pocket/vacant hole)

BP330-385 excitation filter equipped

5-step exchange (4-step filter pocket/vacant hole)

Excitation filter: ø25 mm,

thickness: 6 mm and below

ND filter: ø32 mm,

thickness: 1 mm and below

DM400 (standard)

Slide IN/OUT type

7.4 A

Width: 710 mm

Depth: 740 mm (from the front of tilting tube to

the end of the illuminator)

* Not available in some areas