Olympus Pom Manuel d'instructions - Page 14
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OBSERVATION METHODS:
Standard:
When the instrument is to be used as (standardl
microscope, the
polarizer, test plate, and the Bertland lens are riot necessry.
In principle
those are to be removed from the optical path, but ordinarily the pola-
rizer is left in operation.
Orthoscopic:
With the microscope properly set for this type of the observation,
only the light passing in almost parallel to the optical axis will enter
the view field, thus enabling the user to observe the optical characteris-
tics of the specimen in that direction.
In principle the parallel light source is to be applied, but since this
will darken the field and lower the resolving power of the lens extreme·
Iy, it may be alluminated by allowing the low aperture at the lower
condenser lens only Iswinging out the top lensl.
Therefore, mainly the
low magnification objectives of 20X, or under, are utilized.
It is
reco-
mmended to adjust the contrast by means of the aperLure diaphragm
at the bottom of the condenser in accordance with the objective used.
The centering knob on the objective fixing ring can be utilized to
align the center of the specimen with that of the view field.
Conosopic:
It
is necessary to illuminate the specimen by corn lighting. Put the
top condenser lens back into the position, then attach a high magnifica-
tion objective, such as 40X or lOOX.
After appropriate foc'(ising on the
specimen, insert the Bertland lens and now focus on the interferential
fringe formed at the back focusing plain of the objective lbcteer known
as the conoscopic image}.
by helicoid movement of the tube.
Also a pin.hole cap may be used in place of an eyepiec<: to directly
observe the interferential image mentioned above.
In
this case the
Bertland lens is moved out of the position.
If only a portion of the specimen is of interest to the observer,
he must first remove the Bertland lens, align the center of the stage
with that of the view field, then place the specimen at that center and
trim the field to the desired portion by operating the iris diaphragm.
Put the Bertland lens back into the position, obtain its center by the center-
ing knobs provided, and align it with the center of the ocular field.
Now
it is ready for the observation of the deviation of the interferential
fringe or its measurement by a scaled eyepiece.
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