Olympus U-25ND25 Manuel d'instructions - Page 12

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Dichroic Mirrors and Filter Configurations of Fluorescence Mirror Units

Excitation

Mirror Unit

Method

U-MWU2

U-MNU2

U-MNV2

U-MWBV2

U-MNBV2

U-MWB2

U-MNB2

U-MSWB2

U-MWIB2

U-MNIB2

U-MWG2

U-MNG2

U-MSWG2

U-MWIG2

U-MWIY2

Color Separation Filter Combinations

U-MNUA2

U-MWIBA2

U-MNIBA2

Meaning of Mirror Unit Name

U - M N I B A 2

Mirror unit

Universal

Dichroic Mirror

Excitation Filter

BP330-385

DM400

BP360-370

DM455

BP400-410

BP400-440

DM455

BP420-440

BP460-490

DM500

BP470-490

BP420-480

BP460-490

DM505

BP470-490

BP510-550

DM570

BP530-550

BP480-550

DM565

BP520-550

DM600

BP545-580

DM400

BP360-370

BP460-490

DM505

BP470-550

Model number

For color separation

Excitation (U, UV, BV, B, IB, G, IG or IY)

Bandwidth (SW: Superwide band. W: Wide band. N: Narrow band.)

Barrier Filter

· Autofluorescence observation

· DAPI staining: DNA

BA420

· Hoechest 33258, 33342 staining: Chro-

mosome

· Catechrolamine observation

BA455

· Serotonin observation

· Tetracyine staining: Bones, teeth

· Quinacine & quinacine mustard stain-

ing: DNA

BA475

· Thioflavine S staining: Lymphocite

· Acriflavine staining: Nucleic acid

· CFP

· FITC staining: Fluorescent antibody

· Acridine orange staining: DNA, RNA

BA520IF

· Auramine staining: Tubercle bacteria

· EGFP, S65T, RSGFP

BA510IF

· Rhodamine & TRITC staining: Fluores-

cent antibody

BA590

· Propidium iodide staining: DNA

· RFP

BA580IF

BA610IF

Texas Red staining: Fluorescent antibody

For observing only the U-excitation stain,

BA420-460

when using U-excitation stain together

with FITC.

For observing only the B-excitation stain,

BA510-550

when using B-excitation stain together

with FRITC.

Fluorochrome