Olympus U-BI30-2 Manuel d'instructions - Page 15

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Field iris diaphragm image

Eyepiece field of view

Fig. 6

70%

30%

Aperture iris diaphragm image

Using the Field Iris Diaphragm

{In reflected light brightfield observation:

The field iris diaphragm adjusts the illuminated area in order to obtain

high-contrast images.

Adjust the field iris diaphragm auxiliary lever 4 of the illuminator according

to the objective in use so that the field iris diaphragm image circumscribes

the field of view and block penetration of excess light in the field.

{In reflected light darkfield observation:

Always open the field iris diaphragm fully by pushing in the field iris

diaphragm auxiliary lever 4 all the way.

Centering the Aperture Iris diaphragm (AS)

# The aperture iris diaphragm is locked by a lock screw 6 (Fig. 5) on the

illuminator. Be sure to unlock it by loosen the screw using the Allen

screwdriver (3 mm), provided with the illuminator, before proceeding

to centering.

After completing centering, be sure to lock the diaphragm again to

prevent its malfunction.

1. Press the BF button 1 of the hand switch to select the reflected light

brightfield light path.

2. Press the objective button 3 (Fig. 4) of the hand switch to engage the

10X objective in the light path, place the specimen on the specimen

table and bring the specimen into approximate focus.

}It is recommended to use a highly reflective specimen such as a mirror

to facilitate viewing the aperture iris diaphragm image.

3. Remove an eyepiece, look into the eyepiece sleeve and press the AS-

button 8 of the hand switch to stop down the aperture iris diaphragm to

about 70% opening.

4. If the center of the aperture iris diaphragm is deviated, insert the Allen

screwdriver into the two AS centering screws 7 (Fig. 5) and adjust them

to center the aperture iris diaphragm.

Using the Aperture Iris Diaphragm

{In reflected light brightfield observation:

In general, optimum observation is possible by controlling the aperture

iris diaphragm to between 70% and 80% of the aperture number of the

objective.

{In reflected light darkfield observation:

Always open the field iris diaphragm fully by pushing the AS+ button 9.

}With certain specimens, images with high contrast and little flare can be

observed by stopping down the aperture iris diaphragm below the stan-

dard aperture.

BXFMA

(Figs. 4 to 6)