Olympus BioLever mini Manuel de l'utilisateur - Page 5

Parcourez en ligne ou téléchargez le pdf Manuel de l'utilisateur pour {nom_de_la_catégorie} Olympus BioLever mini. Olympus BioLever mini 5 pages. Micro cantilever

Imprimer la page
Case 5: By placing a piece of paper in front of the photo detector of my AFM sensor head for
studying the position and the shape of the sensor light, several interference light spots are
observed. Those spots align in the direction corresponding to the transverse direction of the
cantilever axis.
Solution:
Adjust the optics of the AFM sensor to locating the primary spot to the center of the
photo detector.
6
Tips for better measurements
1) Merit of using cantilevers with a high resonant frequency
Resonant frequencies of the cantilevers decrease by factor of 4 to 5 in water because of the
water around the cantilevers. The lower resonance cantilever forces operators to wait for a
long time to acquire an image due to a slow scan speed. BL-AC40TS-C2 with a high resonant
frequency of 25 kHz (typ.) in water while being soft with spring constant of 0.1 N/m, enables
the fast imaging of biological samples in water. Of course, scan speed is deeply dependent on
the performance of the scanner and measurement samples. If a small and robust scanner with
high resonant frequency is used, one can expect 5 Hz line scan frequency in AC mode AFM
measurements in water, while 0.5 Hz with conventional silicon nitride cantilevers.
In observing a sparse biological sample on a substrate, a large area,
is scanned to locate a target of the sample. High-speed cantilevers are timesaving device for
AFM measurement particularly in the aiming procedure. Even with a slight improvement in
the scan speed by a factor of two or three, high-speed cantilevers are worth using because they
reduce the time required for observing such large areas before increasing magnification.
High-speed cantilever is a key to improve efficiency of the study in your lab..
e.g.
, a 10 micron square,
- 9e -
2) Merit of low thermal noise cantilevers
Cantilevers vibrate a little in water due to Brownian motion noise. Such noise should be
minimized in force curve measurements for research of folding mechanism of DNAs, proteins
etc. Small size cantilevers show small Brownian motion noise and give good signal noise ratio
in the measurements.
3) Merit of small auto-fluorescence cantilevers
In AFM measurements of biological samples, AFM combined with a fluorescent optical
microscope is useful. After recognizing fluorescently-stained samples with the optical
microscope, a cantilever probe would locate to a point of interests on the sample. Conventional
silicon nitride cantilevers sometimes obstruct fluorescent optical microscopy of the sample
because of the large auto-fluorescent light generated from the cantilever.
This cantilever probe has a far lower auto-fluorescence output which will not obscure your
fluorescent sample.
7
Information
Please contact following if you have any question on this user's manual.
OLYMPUS CORPORATION
Microtechnology R&D Division
2-3 Kuboyama-cho Hachioji-shi Tokyo 192-8512 Japan
Please access to the web page of OLYMPUS micro cantilevers.
http://www.olympus.co.jp/probe
Ver.2.0 June 22, 2009
OLYMPUS CORPORATION
- 10e -