Nikon DS-Ri2 Manuel d'instructions - Page 20

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Tips on Micrographing

4.2

4.2.1

Setting the Environment for Micrographing

4.2.1

When you have attached this product to a microscope, set up the environment for micrographing referring to the following

description.

(1)

Setting Up the Environment

 Adjusting the brightness of the environment

When you micrograph a dark specimen by a fluorescent microscope, the light in the room may come into the

illumination optical path of the microscope; do the micrographing after making the room dark.

Cover the binocular section by the caps to shut the light out.

 Preventing tremor

Micrographing is made with a high resolution so that a little tremor affects the image quality. Place the microscope on

a backlash-free robust desk standing on a stable floor, so that a tremor does not reach the microscope.

You can reduce the impact of tremors by using a vibration isolation table that suits your microscope. Be careful,

especially during micrographing, not to touch the table on which the microscope is placed.

(2)

Setting Up the Microscope Condition

 Using an appropriate filter

For color images:

Insert an NCB filter in the optical path.

For monochrome images:

Insert a filter that suits the photographing purpose in the optical path.

Generally, a GIF (Green Interference) filter achieves a good contrast. Using a filter of the complementary color of the

specimen makes the contrast sharp.

Using a filter

 The way a filter is inserted and removed depends on the microscope. Refer to the manual of your

microscope.

 A third-party color compensation filter (CC filter) can be inserted into the illumination optical path of the

microscope to compensate for changes in color balance caused by the length of the exposure time.

 When using a phase contrast microscope or an interference microscope (two luminous fluxes or multiple

luminous fluxes), you can enhance contrast using a green interference filter (GIF) or a monochrome

interference filter (IF).

 We recommend that you use both an NCB filter and GIF (green interference) filter for DS-Qi2.

 Some microscope model may require a heat-wave absorbing filter.

NCB filter

An NCB (neutral color balance) filter is a color-balancing compensation filter used to adjust color

temperature to daylight values in microscopes that use a halogen bulb as a light source.

Complementary color

For example, green and magenta, red and cyan, and blue and yellow are all pairs of complementary colors.

When complementary colors overlap, their respective hues cancel each other out.

 Setting the field diaphragm

Adjust the field diaphragm so that it circumscribes the viewfield. The field diaphragm has a significant impact on

contrast, especially for fluorescent specimens against dark backgrounds.

Note: For how to adjust the field diaphragm, refer to the instruction manual of your microscope.

Chapter 4

Capturing Images