Nikon A1+ Manuel de l'utilisateur - Page 12

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Image Saturation

1. As well as the image being too dark, it can also be too bright. The confocal

acquires 12bit images. This means that any pixel in the image can have an

intensity value between 0 (black)-4095 (white). The aim is to set the scan

parameters so that the pixels are just below saturation level (4095).

2. In the right hand margin in the software below the

Compact GUI is the LUTs tab. Select the tab and click

on the Pixel Saturation Indicator button. When active this

will colour any pixels in the image that have reached the

4095 maximum intensity value. The colour given to over

saturated pixels can be selected through the down arrow

next to the button. An image window must be open in

order for the Pixel Saturation Indicator button to appear.

3. Images showing the saturation indicator turned on and

off. Saturated pixels in this case have been coloured

yellow.

4. Continue to scan using the Channel Setup option, switch between the detectors

optimising each image so that it is just below saturation intensity level (no or

minimal pixels at 4095 intensity).

If you stop scanning this automatically turns off the Channel Setup option and

pressing Scan again will activate all lasers/detectors.