Nikon A1+ Manuel de l'utilisateur - Page 25

Parcourez en ligne ou téléchargez le pdf Manuel de l'utilisateur pour {nom_de_la_catégorie} Nikon A1+. Nikon A1+ 28 pages. Lbic nikon confocal
Également pour Nikon A1+ : Procédure opérationnelle standard (15 pages)

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Keep image as multchannel- Results in a multiplane tiff file where each frame of the

stack is a channel and displayed in grey scale. The image contains the raw data if the

keep bit depth option has also been selected

Kohler Illumination

1. Select the brightfield optical config

2. Ensure the condenser aperture turret is set to the "A" position

3. Focus the sample

4. Open the condenser aperture fully

5. Close down the field aperture fully

6. If the image of the field aperture is not in sharp focus, adjust the focus

by moving the condenser up/down

7. Centre the image of the field aperture using the centring screws

8. Open the field aperture until you can see the full field of view but no

9. Remove an eyepiece and look down, if you open and close the condenser aperture

you can now see its image

10. Close the condenser aperture until ~70% of the back illuminated

area is filled

11. Reinsert the eyepiece

12.

Kohler should be adjusted whenever you change the lens