Nikon A1R Manuel de l'utilisateur - Page 3

Parcourez en ligne ou téléchargez le pdf Manuel de l'utilisateur pour {nom_de_la_catégorie} Nikon A1R. Nikon A1R 5 pages. Confocal microscope

Step 3.: Power up the lasers that will be used for the current experiment. To prolong the

lifetime of the laser heads make sure to ONLY switch on the lasers that are really needed!

Currently only the 488nm (green) and 647nm (far red) lasers can be controlled

independently. The 647nm (far red) laser can be powered up with a key on the remote on

the laser board. To switch on the 488nm Argon Ion laser switch the "discharge" flip switch

of the controller on the laser board unit to ON with the. The laser will start in about 20

seconds (the output power is about 17-18 mW that will appear on the screen of the

controller.

Step 4.: Switch on the confocal controller that is situated to the left of the microscope. The

switch is on the left side roughly in the middle.

Step 5.: Switch on the stage controller on the table and wait 10 seconds. Waiting is

important as Nikon microscopes are highly customizable and the microscope body checks

which peripheries are currently connected.

Step 6.: Switch on the Microscope body with the RED switch on the optical table.

Step 7.(Optional): If you will use diascopic illumination switch the Halogen lamp on. The

controller is on the table by the microscope.

Step 8.(Optional): If you will use the Epifluorescent illumination switch on the lamp (light

unit is on the table by the microscope). Beware that the lamp needs 15 minutes of cooldown

after being switched of (so check with the previous user). Please also switch off the lamp if

you do not need it within a reasonable amount of time.