Nikon ECLIPSE 80i Manuel d'instructions - Page 35

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BP filter (bandpass filter)

The bandpass filter passes only light of a certain

wavelength, blocking all other wavelengths.

BP filters are used for microscopy of fluorescent

images involving a specific dye in multiple-dye

specimens. (For example, in a double-dye

specimen of FITC and TRITC, the BA520-560 filter

enables microscopy of just the FITC fluorescent

image.)

However, BP filters will not indicate

self-fluorescence, if any (because the fluorescent

image in the above combination is green only). LP

filters are better suited for making fine distinctions

in self-fluorescence based on slight color

differences.

Replacing excitation and barrier filters

18.3

Excitation and barrier filters can be removed from

the filter cube and replaced. (Dichroic mirrors

cannot be dismounted from the filter cube.)

Excitation filters are screw-in filters.

Barrier filters are slide-in filters. Align the

projection on the barrier filter with the groove on

the filter cube and turn clockwise approximately 30

degrees to secure in place.

Individual Operations

18 Selecting Fluorescent Filters

Waveband

emitted by

FITC

Wavelength

Only the FITC fluorescent image is visible.

Turn about 30° to secure in place.

Excitation

filter

BA520-560 (BP type)

Waveband

emitted by

TRITC

Barrier filter