Amersham Pharmacia Biotech M-925 Petunjuk Manual - Halaman 2
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Halaman Cetak2 Mount the mixer in an an ÄKTAdesign
chromatography system, or together with
an ÄKTAdesign P-900 series system pump
in stand-alone applications. See the
ÄKTAdesign user documentation for
further information.
3 Fit a mixer chamber to the mixer housing
as described below.
Fitting mixing chamber
WARNING! When using hazardous
chemicals, ensure that the mixer chamber
has been flushed through with distilled
water before removing an used chamber.
A mixer chamber must be fitted to the mixer
housing prior to use.
0, 6 m l
2 m l
Mixer chambers of 0.6, 2, 5 and 12 ml are
available.
Recommended flow rates for each mixing
chamber are specified in the table below.
When using eluents that are more difficult to
mix such as Isopropanol and water, a large
mixer volume can be used to get optimum
mixing.
2
1
2 m l
5 m l
Mixing
Recommended flow rates
chamber
Two pump
volume
gradients
and max.
pressure
0.6 ml/25 MPa 0.001-10.0 ml/min 0.1-5 ml/min
2 ml/25 MPa
0.5-100 ml/min
5 ml/25 MPa
5-100 ml/min
12 ml/10 MPa –
For BufferPrep select flow rates as for switch valve
gradients.
Outlet
Spring
Chamber lock
0 0 , , 6 6
Inlet
To fit a mixer chamber:
1 Open the plastic lock covering the mixer
chamber compartment. A spring is
securing the mixer chamber in position
when the lock is opened.
2 Insert a mixer chamber, and close the
lock.
3 Fit the inlet and outlet
tubing.
Switch valve
gradients
1-10 ml/min
5-30 ml/min
15-100 ml/min
Inlet
M -
9 2
5
Mixer M-925
Instructions 56-8101-00 Edition AC