Dagan IX2-700 Panduan Pengoperasian - Halaman 5

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IX2-700 Dual Intracellular Preamplifier

CONTROL DESCRIPTION

3.5 DIGIT LCD METER

The meter has five functions. They are:

(1)

Measuring the Channel 1 output voltage at a gain of one (i.e. I Vm)

(2)

Measuring the Channel 2 output voltage at a gain of one (i.e. 1 Vm)

(3)

To measure the potential difference between Channel 1 and Channel 2.

(4)

To measure the membrane current Im from Channel 1 scaled by the

headstage for automatic decimal point on the meter.

(5)

To measure the membrane current Im from Channel 2 scaled by the

headstage for automatic decimal point on the meter.

BUZZ OPERATION

The buzz or tickle function contained in the IX2-700 is used to impale an

electrode in the cell. The IX2-700 has a controlled buzz magnitude. Start with the

Buzz Magnitude in the counterclockwise or minimum position and increase while

initiating buzzes until you achieve desired cell penetration. (Buzz magnitude is a

trimpot located under the buzz switch).

CAPACITANCE COMPENSATION CONTROL

This control provides input capacitance neutralization. Without this

compensation high frequency signals from a high impedance source such as a

micropipette would be lost because of stray capacitance at the input. Start with this

control at its minimum in the counterclockwise position.

The amount of capacitance compensation available depends on the

capacitance compensation range ordered with the probe you are using. Ranges as

small as 15 pf and as large as 100 pf are available (35 pf standard). Smaller ranges

produce less noise and are ideal for very low capacitance microelectrodes used

with high switching frequencies. The larger capacitance compensation ranges are

ideal for doing typical bridge preamplifier procedures and also switched single

electrode voltage clamping in deep tissue, where the microelectrode has a high

capacitance associated with it.