BD FACSJAZZ Panduan Pengguna - Halaman 8
Jelajahi secara online atau unduh pdf Panduan Pengguna untuk Peralatan Laboratorium BD FACSJAZZ. BD FACSJAZZ 9 halaman. Satchmo stanford shared facs facility
Halaman CetakThe Sheath Tank is not pressurizing
1. Ensure that the pressure release valve is closed.
2. Reinstall the sheath tank cover, feel and listen to be sure it is seated properly.
3. The large black gasket may need to be re-lubed, please ask for help.
Removing Bubbles from the System Lines
1. Check to make sure the sheath line filter is still in working condition.
2. Try purging and pulsing with ALCOHOL in the de-bubbler. Make sure to rinse the nozzle with WATER
after the alcohol.
I got a clog and I have BSL-1 Samples—You may need to get staff for help
1. Push STREAM to stop the stream.
2. Remove your sample from the sample port.
3. Remove your collection tubes/plate from the collection area.
4. Remove the nozzle and place it in a tube of water.
5. Sonicate the nozzle.
6. Screw the nozzle back into the nozzle assembly.
7. Place the sink under the nozzle assembly.
8. Put a tube of ALCOHOL the instrument a press the OVERRIDE button. Wait for the alcohol to start
to drop from the nozzle.
9. Fill the de-bubbler with ALCOHOL.
10. Place the de-bubbler back under the nozzle with ALCOHOL. Lower the nozzle so that it is sitting in
the ALCOHOL press PURGE and then PULSE in the software.
11. Remove the de-bubbler from under the nozzle and dump out any leftover fluid.
12. Place a tube of WATER on the instrument and press OVERRIDE button and wait for the water to
drip from the nozzle.
13. Fill the de-bubbler with WATER.
14. Place the de-bubbler back under the nozzle with WATER. Lower the nozzle so that it is sitting in
the WATER press PURGE and then PULSE in the software. Do this until you no longer see bubbles
coming from the nozzle assembly.
15. Remove the sink from under the nozzle assembly.
16. Push the STREAM button in the software to start the stream.
17. Push the BACKFLUSH button and allow the system to backflush for 10-15 secs.
18. Re-run the 197 beads and the Accudrop beads.
19. Re-target your collection tubes.
20. Filter your sample again, run your sample and check your gates.
21. Start sorting again.
I got a clog and I have BSL-2 Samples—You may need to get staff for help
1. Do NOT touch your sample or collection tubes
2. Turn the ON the Aerosol Management, and increase the suction to 100%, Wait 5 minutes allowing it to
remove any potentially hazardous materials.
3. Follow steps 1-21 of "I got a clog and I have BSL-1 Samples"
lasted updated by MW on 11/10/2017
Troubleshooting
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