BD FACSAria Prosedur Startup, Qc & Shutdown - Halaman 3
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2. Fluidics & Stream Startup
1
Fill sheath tank and empty waste.
1
Sheath=1xPBS. To prepare, combine 200 mL of 10XPBS with 1800 mL of dH2O.
For Aria-7:
Tue-Fri: No fluidic startup needed. Remove waste container and add bleach to bring it to a final
concentration of 10% and leave it to the side. Connect separate empty waste container for startup.
Sat-Mon: Fluidic startup is needed, empty the waste into designated EtOH disposal tanks after startup is
completed and connect the empty waste container to the sorter.
For Aria-2/4: at the start of each day empty the waste into the designated EtOH disposal tanks. No
bleach should be added. Fluidic startup is done every day.
1
Change connections between EtOH tank and Sheath Fluid tank to prepare for sheath
2
startup.
Fluidics setup for EtOH shutdown.
1. Fluidics line hooked up to
1
designated EtOH filter.
2. Pressure line hooked up to
2
male connection on EtOH tank.
3. Release valve down, allowing
3
pressurization of EtOH tank.
Fluidics setup for Sheath startup.
1. Fluidics line hooked up to
4
designated sheath filter.
2. Pressure line hooked up to
5
male connection on Sheath
tank.
3. Release valve down, allowing
6
tank to pressurize with fluidics
startup.
*To Refill tank when empty,
disconnect pressure line and open
release valve to depressurize
sheath tank and fill with 1x PBS.
1
Startup fluidics.
3
Go to Cytometer Fluidics Start-up. Follow on screen prompts.
EtOH tank connected
3
1
EtOH tank disconnected
Sheath tank disconnected
2
Sheath tank connected
5
6
4