ATLAS BIOTECNOLOGIES AB100 Buku Petunjuk - Halaman 7

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and the preset volume) exceeds the permissible value

given in the table in section 1, the pipette recalibration

procedure should be carried out.

Before starting the recalibration it is necessary to check

whether the following requirements have been fulfilled

during error determination:

• the ambient temperature, and the temperature of the

pipette, tips and water was identical

• the density of the liquid used is close to that of distilled

water

• the balance with appropriate sensitivity has been used.

Volume checked [μl]

0,1 - 10

10 - 100

> 100

• mg/μl conversion factor has been taken into ac count

• the requirements given in sections 3 and 7 have been

fulfilled

If the above conditions are satisfied and the ac cu racy error

for selected volume given in section 1 exceeds the per-

missible value, the pipette recalibration procedure

should be carried out.

The recalibration can be performed within one full turn

of the key to the right or to the left only.

Recalibration conditions:

• Ambient temperature and the temperature of the

pipette, tips and liquid should be within the range 20-

25°C and stabilised during weighing within ±0.5°C

• Measurements should be conducted using distilled

water

• Balance sensitivity should be suitable for the vo lume to

be controlled

Recalibration procedure:

• Set the dose volume depending on the pipette volume

according to the following table:

Balance sensitivity [mg]

≤ 0,001

≤ 0,01

≤ 0,1

Range

Preset

Model

of the pipette

volume

volumes [μl]

[μl]

AB2

0.1 - 2

0.2

AB10

0.5 - 10

0.5

AB20

2 - 20

AB50

5 - 50

AB100

10 - 100

AB200

20 - 200

AB250

50 - 250

AB1000

100 - 1000

100

AB5000

500 - 5000

500

AB10000

1000 - 10000

1000

• Perform 5 aspirations, weigh each one and calculate

the average value of the aspirations.

• Calculate average aspirated volume in [μl] multiplying

the average aspiration amount [mg] by the distilled water

density coefficient [μl/mg], which depends on tempera-

ture and pressure according to the following table:

Temperature [°C]

95.0

1.0028

1.0030

1.0032

1.0034

1.0037

1.0039

If the average aspirated volume exceeds the per missible

value, the following should be done:

• Remove the pipetting pushbutton, (Fig. 4A),

Warning: The pipetting pushbutton consists of 2 parts:

the knob (Fig. 1A2) and the pushbutton (Fig. 1A1). After

removal of the pushbutton, both parts are separated.

• Holding the volume setting knob to protect it against

rotation, insert the calibration key into the cuts of the

calibration screw, (Fig. 4B),

•Turn the key clockwise to reduce the aspirated volume,

or counter-clockwise to increase the volume, (Fig. 4C).

ENGLISH

Volume change

ΔV for full turn

Permissible

of the

volumes

calibration key

[μl]

(24 increments)

0.176 - 0.224

0.06

0.48 - 0.52

0.33

1.94 - 2.06

0.63

4.875 - 5.125

2.50

9.84 - 10.16

2.50

19.76 - 20.24

6.30

49.5 - 50.5

6.30

98.4 - 101.6

25.00

494 - 506

125.00

975 - 1025

250.00

Pressure [kPa]

101.3

105.0

1.0029

1.0031

1.0033

1.0035

1.0036

1.0038

1.0040