ATLAS BIOTECNOLOGIES AB1000 Buku Petunjuk - Halaman 6

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some time to follow the change of pressure by holding

the pipette tip in its position for at least 2 sec. after the

aspiration and the blow out stroke.

If in extreme cases this method of operation does not

result in accurate values, a compensation could be

achieved as follows:

Weigh the liquid pipetted when the pipette is set to the

nominal value. Then calculate the set-off from the nomi-

nal value:

Corr: val. = 2 x nom. val. -

- weight of the sample

- density of liquid

Check this operation once again and correct if neces-

sary. Note the corrected value for further pipetting the

same kind of liquid.

6 - PIPETTE TIPS

A.B.T. tips are made from high performance polypropy-

lene and their quality guarantees the precision and accu-

racy associated with the pipette. Strict control is main-

tained throug hout the manufacturing process to ensure

the highest quality.

The use of inferior quality tips will seriously degrade the

performance of the pipette.

Tips 10

These tips are used for volumes between 0.1 μl and 10 μl.

They are used with the AB2 and AB10 models.

Tips 200

These tips are used for volumes between 2 μl and 200 μl.

They are used with the AB20, AB50, AB100 and AB200

models.

Tips 300

These tips are used for volumes between 50 μl and 250 μl.

They are used with the AB250 models.

Tips 1000

These tips are used for volumes between 100 μl and

1000 μl. They are used with the AB1000 models.

Tips 5000

These tips are used for volumes between 500 μl and

5000 μl. They are used with the AB5000 models.

Tips 10000

These tips are used for volumes between 1000 μl and

10000 μl. They are used with the AB10000 models,

which are equipped with a white pushbutton.

7 - RECOMMENDATIONS

Observing the following recommendations will ensure

maximal possible accuracy and precision of liquid sam-

pling.

• Make sure to operate the pipette slowly and smoothly.

• The depth of immersion in the sample liquid should be

the minimum necessary and should remain constant

during aspiration.

• The pipette should be held in a vertical position.

• Change the tip when volume setting is chan ged or when

a different liquid is to be aspirated.

• Change the tip if a droplet remains on the end of the tip

from the previous pipetting operation.

• Each new tip should be pre-rinsed with the liquid to be

pipetted.

• Liquid should never enter the pipette shaft. To prevent

this:

- Press and release the pushbutton slowly and smoothly.

- Never turn the pipette upside down.

- Never lay the pipette on its side when there is liquid in

the tip.

• Never force the volume setting beyond its recommended

limits.

• When pipetting liquids with temperatures different from

the ambient temperature, it is reco mmended to pre-

rinse the tip several times before use.

• Do not pipette liquids with temperatures above 70°C.

• When pipetting acids or corrosive solutions which emit

vapours, it is recommended to disassemble the shaft

and to rinse the piston and seal with distilled water after

finishing the pipetting operation.

8 - RECALIBRATION

Pipettes are calibrated by gravimetric me thod, using tips

and distilled water, at the temperature 20±1°C, accord-

ing to EN ISO 8655 standard.

If during pipette operation you find that the accuracy

error (the difference between the real aspirated volume

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