Beckman Coulter Cytomics FC 500 Panduan Pengguna - Halaman 12
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Routine Cleaning for All users after each use:
(please note: feel free to log out of RIMS before washing and/or shutdown – you
should only be charged for time you spend with your research, not cleaning!)
1. After finishing your data acquisition, clear the worklist by clicking
should see the Acquisition Manager clear. (If the Acquisition Manager is not
visible, use "View" and select Acquisition Manager.)
2. Be sure that the software is in carousel Automatic mode. The cytometer is in
carousel Automatic mode when the message "Set Automatic Mode" appears in
the lower right corner of the software window.
a. If samples were being run in Single Tube mode (
click
b. If samples were being run in MCL Manual mode (
shaded), click
3. Insert a new panel by clicking
4. In the window that appears, click on the drop-down list next to "Look in" and
click on "Users." Then click on "FLOW," then "Panel," then "cleansepanel".
Click "Open"
1. This will put 4 lines into the Acquisition Manager window. In the "Carousel No."
column, type in the number of the carousel being used (1 or 2) and push Enter.
Ensure that the "Location" in the next column fills in with 1, 2, 3 and
4. See example to the right ------>
2. Put about 2mL of 10% bleach solution into a sample tube
3. Put about 2mL of distilled water into each of three sample tubes
4. Place the bleach tube in position 1 of the carousel; place the water
tubes in positions 2, 3 & 4
5. Put the carousel into the MCL and close the MCL cover
6. Click
7. Wait. The software may ask if the protocol that was previously being run should
be saved; answer Yes or No as desired.
8. Wait for the cytometer to automatically run all 4 tubes. It will take about 10
minutes.
again to take it out of Single Tube mode.
again to take it out of MCL Manual mode.
You
clicked and shaded),
clicked and