Drummond Nanoject II Buku Petunjuk - Halaman 4

Jelajahi secara online atau unduh pdf Buku Petunjuk untuk Unit Kontrol Drummond Nanoject II. Drummond Nanoject II 12 halaman. Digital control box

1) Plug the injector cable into the "HEAD" port on the right side of the control box.

2) If using the footswitch, insert the footswitch cable into the "FOOTSWITCH" port on

the right hand side of the control box.

3) Plug the control box into a standard electrical outlet using the appropriate

adapter head on the power supply.

4) Turn on unit with the on/off switch located on the left hand side of the control box.

Initially the Drummond logo will be displayed on the screen followed shortly by the

Operational Mode screen.

5) Micropipette Pulling and Backfilling

The Nanoject II requires the use of pulled micropipettes from the glass provided.

Ideally, the tip size should be 10-30 microns in size. The capillary glass provided is

produced from N-51-A material and has a softening point of 780°C. Many researchers

pull the tips and then break them off with forceps. This enables piercing the cell

membrane much easier with no skipping. Once the tips are pulled, they must be

"backfilled" with oil (or other non-compressible fluid) before attachment to the injector.

Silicone or mineral oil is frequently used. Backfilling is facilitated by using the 30 gauge

x 2˝ needle and a syringe. Disposable spinal needles are also frequently used.

NOTE: THE NANOJECT II WILL NOT OPERATE PROPERLY WITHOUT

BACKFILLING THE MICROPIPETTE.

6) Securing the Micropipette to the Injector Standard Collet/O-ring (Fig. 1)

Once the micropipette is backfilled, loosen the collet. The pointed wire plunger should

be positioned so you can just see the tip flush with the end of the collet (slightly

recessed is also acceptable). This is referred to as the "Home" position. Push them

micropipette onto the wire plunger and as you push the tip on, feel it go through the

large O-ring and seat in the white spacer. Once positioned, tighten the collet securely.

Give the micropipette a pull to confirm it is securely mounted. See Fig. 1 for proper

configuration of the O-rings and the white spacer. It is absolutely essential that these

components are properly configured.

NOTE: THE WHITE SPACER HAS ONE FLAT SIDE AND ONE SIDE WITH A

RECESS MACHINED AROUND THE HOLE. THIS RECESS IS TO RECEIVE THE

BACK END OF THE PIPETTE.

Quick Start Guide