Automate ValveLink 8 Panduan Pengguna - Halaman 7

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Perfusion Pressure Kit

Brass input connector hose barb size 1/4" i.d.

Max. input

pressure 100 psi!!

5 micron bowl fi lter.

Press bottom button to empty.

Syringes may also be placed in a water

bath for temperature control.

2-way stopcocks and lure-lock to 1/16" hose fi ttings are

included for each channel to connect syringes to hose.

Hoses should continue from syringes to valves

(sold separately #09-04, -08 & -16)

Press regulator knob down to lock.

Pull up to unlock.

Individual air lines can be

adjusted or turned-off.

Be very careful with these

plastic hose connections.

Multi-barrel Perfusion Pencil

#04-04-xxx

4-into-1 Pencil with tip

#04-08-xxx

8-into-1 Pencil with tip

#04-16-xxx

16-into-1 Pencil with tip

(xxx = 100, 250, or 360 µm)

Epoxy or silicon glue may be needed to

completely seal connections between valve

tubing and the manifold tip in-ports.

Tighten removable tip for

minimum dead volume.

One of the eight lines may be connected to vacuum through a valve

programmed to suck the dead-volume clear between solutions.

Chemical Information

The tubing inside the manifold body is polyimide (nylon). The largest

connection tubing is vinyl. The Removable tips include a medical grade

polypropylene Luer-lock fi tting with a fused silica (quartz) needle coated with

polyimide. These materials are resistant to most acids, bases and organic solvents.

To avoid dust contamination, we recommend pre-rinsing the Perfusion Pencil and

Tip with distilled water. Also, it is good practice to discard the fi rst few microliters

of solution before using the device. The Perfusion Pencil and Removable Tips are

shipped non-sterile. They can be chemically sterilized or autoclaved. However,

repeated autoclaving may weaken the adhesive bond between the Lure-lock fi tting

and the needle.

Maintenance

You can expect several years of useful lifetime for your tip if you wash it daily. Use

a syringe or vacuum to pull fi rst water, then alcohol three times each through the

tip. If the Multibarrel Perfusion Pencil is fi lled with pure salt solution, leaving it in

the syringe overnight will not usually cause the tip to block. However, if it is used

for high viscosity fl uids, fl ushing after each use is recommended.

Cutting

The Removable Tips are shipped with 1.5" (3.8 cm) length polyimide needles. They

are intended to be cut to a desired length by rolling a razor blade on them against

a hard surface. However, this may leave a small crack or barb on the tip. To get a

fl at cut, score the coating of the needle with a ceramic cleaving stone or a diamond

cutter and pull directly apart, making sure not to pull at an angle. You may notice

a larger outer tube enclosing the lower part of our smaller, 100µm needles. This is

simply for added rigidity, and can be cut if needed.

™

(sold separately)

Replacement Tips:

Part No. Description

Description

04-xxx

specify

100, 250, or 360 µm