Olympus 1X71 Panduan Pengguna - Halaman 4

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General specifications

Microscopy techniques available
Brightfield
Phase contrast
Epitluorescence
Objectives
O l y m p u s U P l a n F L N 1 0 x P h 1 N A 0 . 3 W D 1 0 . 0 m m
Olympus LUCPlanFLN 20x Ph1 NA 0.45 WD 6.6 -7.8 with correction collar
Olympus LUCP lanFLN 40x Ph2 NA 0.6 WD 3.0 - 4.2 with co rrection collar
Z e i s s o b j e c t i v e s c a n a l s o b e u s e d
Filter cubes (see p11-15)
Position Filter set name
1
DAPI
2
GFP
3
DsRed (TRITC/Cy3)
4
Cy5
5
Cy7
6
empty (brightfield)
Camera (see p16)
Hamamatsu ORCA R
Fluorescence illumination (see p16)
Prior Lumen 200 Metal Halide Light Source (2000 hours/bulb)
Pixels to Microns calibration
5x objective binning 1x1
10x objective binning 1x1
20x objective binning 1x1
40x objective binning 1x1
Calibration with a micrometer under transmitted white ligh t
Consumables list
Price correct as of November 2013
Prior Lumen 200 bulb LM375 (£550, Prior Scientific Instruments Ltd)
Prior Lumen 200 light guide LM587 (£400, Prior Scientific Instruments Ltd)
Halogen bulb 12V/100W (£1.8, Technical Lamp Supplies UK)
Exciter
350/50x
ET470/40x
ET545/30x
ET620/60x
ET710/75x
2
CCD Camera with HClmage Capture Software
1 pixel = 1.88 um
1 pixel = 1.03 um
1 pixel = 0.514 um
1 pixel = 0.256 um
Beamsplitter Emitter
400LP
ET460/50m
495LP
ET 525/50m
T570LPXR
ET620/60m
T660LPXR
ET700/75m
T760LPXR
ET810/90m
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