Olympus 1X71 Panduan Pengguna - Halaman 4

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Halaman Cetak

General specifications

Microscopy techniques available



Brightfield



Phase contrast



Epitluorescence

Objectives



O l y m p u s U P l a n F L N 1 0 x P h 1 N A 0 . 3 W D 1 0 . 0 m m



Olympus LUCPlanFLN 20x Ph1 NA 0.45 WD 6.6 -7.8 with correction collar



Olympus LUCP lanFLN 40x Ph2 NA 0.6 WD 3.0 - 4.2 with co rrection collar



Z e i s s o b j e c t i v e s c a n a l s o b e u s e d

Filter cubes (see p11-15)

Position Filter set name

DAPI

GFP

DsRed (TRITC/Cy3)

Cy5

Cy7

empty (brightfield)

Camera (see p16)

Hamamatsu ORCA R

Fluorescence illumination (see p16)

Prior Lumen 200 Metal Halide Light Source (2000 hours/bulb)

Pixels to Microns calibration

5x objective binning 1x1

10x objective binning 1x1

20x objective binning 1x1

40x objective binning 1x1

Calibration with a micrometer under transmitted white ligh t

Consumables list

Price correct as of November 2013



Prior Lumen 200 bulb LM375 (£550, Prior Scientific Instruments Ltd)



Prior Lumen 200 light guide LM587 (£400, Prior Scientific Instruments Ltd)



Halogen bulb 12V/100W (£1.8, Technical Lamp Supplies UK)

Exciter

350/50x

ET470/40x

ET545/30x

ET620/60x

ET710/75x

CCD Camera with HClmage Capture Software

1 pixel = 1.88 um

1 pixel = 1.03 um

1 pixel = 0.514 um

1 pixel = 0.256 um

Beamsplitter Emitter

400LP

ET460/50m

495LP

ET 525/50m

T570LPXR

ET620/60m

T660LPXR

ET700/75m

T760LPXR

ET810/90m