Olympus BHS Buku Petunjuk - Halaman 17

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4. Color and B & W Photomicrography

a) Color photomicrography

Voltage setting

........

Within a range as marked with the letters "PHOTO" on

the voltmeter.

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vu sara é Insert the LBD-2 filter into the filter mount at the light

exit on the microscope base.

(in case of daylight film)

Lightintensity

........

Should

be adjusted by means of the ND filters rather

than by means of voltage change.

The

range

of voltage setting

mentioned

above

indicates a guide line for color photomi-

crography.

If the

photograph,

taken

this way,

however,

gives

bluish

tint, lower the

voltage slightly, and if it gives reddish tint, raise the voltage slightly.

b) Use of contrast filters for B & W photomicrography

Green filter

.....

This filter is most recommended, since it aids in obtaining photo-

micrographs of highest resolution and maximum contrast.

Recommended as contrast filter when it is desirable to emphasize

the blue color in the stained portion of a specimen.

Cobalt filter

....

Not

generally

recommended

for

photomicrographic

purposes

since it reduces both resolution and contrast.

Use it for specific

applications only when

it is desirable to emphasize yellow in the

stained portion of a specimen.

Nofilter

.......

A photograph is taken with less contrast than with the green filter.

5. Focusing Procedure

Use the field of view eyepieces for focusing on the film plane.

Each

field of view eyepiece

has a focusing front lens and a reticle with 4 frames, each frame indicating the area covered

by a specific power NFK

photo eyepeice.

(Fig. 22).

The

number

at each

frame

indicates

the

magnification

the

photo

eyepiece.

The

image

in the field of view eyepiece and the

image

the film

plane are in focus at the

same

time.

Several

type

field of view eye-

pieces

are

available,

according

the film

size employed.

Fig. 22

Field of view eyepiece | 35WHK10X | PWHK10X

4X5WHK10X | MHWHK10X

35mm Back | 3% x 4%"

4"x 6"

16mm Bolex

Polaroid

Sheet Film or

camera

Acme camera

Back

Polaroid Film | 120 Roll Film

Holder

1) Select the field of view eyepiece matching the camera back in use and insert it into the

right eyepiece tube of the trinocular tube, aligning locating groove and

locating pin.

2) While

looking through

the field of view eyepiece, rotate the eyepiece front lens in screw

mount

to focus on the double cross lines in the field.

For sharp focusing with objectives

4X or lower, the focusing magnifier FT is recommended

3) Bring

the

specimen

detail to be photographed

within

the

frame

corresponding

to the

power of the NFK eyepiece in use and focus on the specimen with the microscope fine

adjustment

knobs.

Make sure the light path selector knob on the observation

tube is

either on the white

(V) or yellow-green

(CV)

band.

4) It is recommended

to tighten

the tension adjustment

ring considerably

to prevent the

stage from

dropping during long exposures.