Olympus BHSP Buku Petunjuk - Halaman 16

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1) Aperture iris diaphragm

Adjust the opening of the aperture iris diaphragm ac-

cording to the various conditions such as the numerical

aperture of the objective, image contrast, depth of focus,

70-80%

and flatness of field. Generally i t is often preferable to

30-

20%

stop down the aperture iris diaphragm t o about

70%

80%

of the N.A. o f the objective.

After the eyepiece is removed from the observation

tube, if necessary, look through the observation tube

and check the opening of the aperture diaphragm at the

objective pupil.

2) Field iris diaphragm

The field iris diaphragm controls the diameter of the ray bundle impinging on the specimen

surface and thus increases image definition.

Generally, i t is preferable t o slightly increase the diameter of the field iris diaphragm until

it is just outside the field of view.

2. Pre-focusing Lever

This lever

is provided to prevent possible

contact between specimen and objective as

well as to simplify coarse focusing. (Fig.

16)

The lever is locked after coarse focus has

been accomplished. This prevents further

upward travel of the stage by means of the

coarse adjustment knobs, and automatically

Focus Adjustment

1 . Tension of Coarse Adjustment Knobs

and Fine Adjustment

Although the tension of the coarse adjust-

ment knobs has been already adjusted for

optimum performance by the manufacturer,

i t is possible to personally adjust the tension

o f the coarse adjustment for either heavy or

light movement depending on the operator's

provides a limiting stop if the stage is low-

ered and then raised again. The pre-focusing

lever does not restrict fine focusing.

- .

Fig. 16

preference by rotating the tension adjust-

Fig.

ment ring

(Fig. 15)

The ring can be rotated by inserting a screwdriver into one of the holes on the periphery of

the ring. The clockwise rotation (in the direction of the arrow) tightens the coarse adjust-

ment knobs. Do not loosen the ring too much, because the stage may drop or the fine

adjustment knobs may slip.

NOTE: Do not rotate the right and left coarse adjustment knobs in the opposite directions

simultaneously. If the stage drops and the specimen goes out of focus, the tension

adjustment ring is too loose. Tighten the ring.