Olympus BX-RFAA Petunjuk Manual - Halaman 30

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Exciter Balancer U-EXBABG/EXBAUB/EXBAUG (for exclusive use with the BX-RFA/RFAA)

}When a fluorescence image obtained by multiple excitation of U/B/G is observed with dual- or triple-band fluorescence

mirror units, use the exciter balancer to select the balance between the excitation light intensities of the fluorochromes.

* To use an exciter balancer with the BX-RFAA, remove the adjustment lever and attach it to the opposite end.

Installation Procedure (Fig. 9)

U-EXBAUG

Fig. 9

Operation Procedure

Observing a Double Stained Specimen

1. Set up normal reflected fluorescence observation.

2. Mount the fluorescence mirror units for double staining and engage them in the light path.

}Olympus standard products

Exciter Balancer

Fluorescence Mirror

Units for Double Staining

U-EXBABG

U-EXBAUB

U-EXBAUG

1. Stand the adjustment lever @ of the exciter balancer vertically and insert

it the ND filter insertion slot with the same number as the slider on the

rear part of the right side of the illuminator.

· The insertion position is variable depending on the type of the exciter

balancer.

· Always insert the exciter balancer so that the clamping screw faces to-

ward the rear.

2. Tighten the clamping screw using the Allen screwdriver.

U-EXBABG

U-EXBAUB

Fluorescence Mirror Units

Fluorescence Mirror

Units for Triple Mounting

· U-DM-FI/TR2

· U-DM-FI/PI2

· U-DM-FI/TX2

· U-DM-DA/FI/TR2

· U-DM-DA/FI2

· U-DM-DA/FI/PI2

· U-DM-DA/FI/TX2

· U-DM-DA/TR2

· U-DM-DA/PI2

· U-DM-DA/TX2

Clamping screw

Adjustment lever*

# Due to its characteristics, the G-excitation

has a narrower intensity control range than

the U- and B-excitation. The intensity con-

trol range is also variable depending on

the status of specimen and variance in

mirror units' characteristics.

# Lighting irregularities may be observed on

the upper and lower edges of the field dye

to the rotation angles of filters and the vari-

ance in mirror units' characteristics. How-

ever, these lighting irregularities do not af-

fect the photographed area.