Olympus BX-RFAA Petunjuk Manual - Halaman 9

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REFLECTED FLUORESCENCE OBSERVATION

PROCEDURE

}For the operating procedures for simultaneous observations of phase contrast or transmitted Nomarski DIC observation

with fluorescence observation, see chapter 4, "SIMULTANEOUS FLUORESCENCE OBSERVATION METHODS" (page 15).

· Attach the fluorescence mirror units and objectives which are suitable for the observation method to be used.

· Center the mercury burner.

· Set the main switch @ of the BX-UCB control box.

Set the main switch to " I " (ON) and wait until

the arc image stabilizes (5 to 10 minutes).

Place the specimen on the stage.

Engage the mirror unit matching the speci-

men.

Engage the objective in the light path and bring

the specimen in focus.

Adjust the collector lens so that the field of

view is uniform and brightest.

Start observation.

}Engage the shutter if you interrupt observation for a short time.

Preparation

Engage a ND filter in the light path

as required.

Adjust the field iris diaphragm.

Adjust the aperture iris diaphragm.

(Controls Used)

² Main switch

³ Specimen holder

| X/Y-axis knobs

ƒ MU+ (Mirror Unit Right) or

MU- (Mirror Unit Left) button

... Objective button

† RSHT (shutter) button

‡ Stage UP/DOWN button

Š ND filter

‰ Collector lens focusing knob

‹ Field iris diaphragm knob

Œ Aperture iris diaphragm knob

† RSHT (shutter) button

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(P. 13 - P. 14)

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