Olympus BX53 Petunjuk Manual - Halaman 30

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5-6 Immersion Objectives

Be sure to use the provided Olympus Immersion oil.

CAUTION

5-7 Objectives with Correction Collar

Fig. 43

Fig. 44

Using Immersion Objectives

1. Focus on the specimen with objectives in the order of lower-power to

higher-power ones.

2. Before engaging the immersion objective, place a drop of provided

immersion oil onto the specimen at the area to be observed.

3. Turn the revolving nosepiece to engage the immersion objective, then

focus using the fine adjustment knob.

Since air bubbles in the oil will affect the image quality, make

CAUTION

sure that the oil is free of bubbles.

a. To check for bubbles, remove the eyepiece and fully open the field and

aperture iris diaphragms, then look at the exit pupil of the objective inside

the observation tube. (The pupil should appear round and bright.)

b. To remove bubbles, turn the revolving nosepiece to repeatedly defocus

and refocus the oil immersion objective.

} If the condenser engraving shows a numerical aperture (NA) of 1.0 or

higher, the number applies only when oil is applied between the slide

glass and the top surface of the condenser. When oil is not present, the

NA is about 0.9.

4. After use, remove immersion oil from the objective front lens by wiping

with gauze slightly moistened with absolute alcohol.

Caution in use of immersion oil

CAUTION

If immersion oil enters your eyes or contacts with your skin, imme-

diately take the following treatment.

Eyes: Rinse with fresh water (for 15 minutes or more).

Skin: Rinse with water and soap.

If the appearance of the eyes or skin is altered or pain persists,

immediately see your doctor.

} If the thickness scale setting of the objective with collection collar does

not match the cover glass thickness, the objective cannot manifest the full

performance. To prevent this, be sure to perform the following adjustment

when using an objective with collection collar.

Adjustment Procedure

· If the cover glass thickness is known, set the correction collar @ to that

scale value. (Fig. 44)

· If the cover glass thickness is unknown, adjust the correction collar @

and fine adjustment knob alternately until the positioning with the highest

contrast is obtained.

} Be careful not to touch the correction collar @ when turning the revolving

nosepiece.

(Fig. 43)