Olympus CKX41 Petunjuk Manual - Halaman 13
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Halaman Cetak
- 1. Table of Contents
- 1. General Precautions for Observation
- 1. Using the Controls
- 2. Important
- 2. Applicable Fluorescence Objectives
- 3. Nomenclature
- 3. Centering the Mercury Burner
- 4. Controls of each Module
- 4. Switching the Filter Slider
- 4. Troubleshooting Guide
- 4. Specifications
- 4. Assembly
- 4. Assembly
- 4. U-Excitation Filter Set Ckx-Nu (Optional)
- 5. Summary of Reflected Fluorescence Observation Procedure
- 5. Proper Selection of the Power Supply Cord
2
Selecting the Reflected Light Fluorescent Mirror
}The reflected light fluorescent mirrors for B-excitation and G-excitation have been installed in the
fluorescent mirror slider at the factory.
If required, the optional CKX-NU mirror for U-excitation can be mounted in the open position or by
removing the B- or G-excitation mirror. (Work to be done by the dealer)
Fluorescent mirror specifications
Excitation Fluorescent Mirror Dichroic Mirror Excitation Filter
B
G
U
CKX-NU
Spectral characteristics
DM500
BP460-490C
DM570
BP480-550C
DM400
BP360-370
B-excitation
Wavelength nm
U-excitation
Wavelength nm
REFLECTED FLUORESCENCE SYSTEM
Barrier Filter
· FITC: Fluorescent antibody method
· Acidine orange: DNA, RNA
BA520 IF
· Auramine: Tubercle bacillus
· EGFP, S65T, RSGFP
· Rhodamine, TRITC: Fluorescent anti-
body method
BA590
· Propidium iodide: DNA
· RFP
· Auto-fluorescence observation
· DAPI: DNA
BA420
· Hoechest 33258, 33342: Chromosome
G-excitation
Wavelength nm
FOR CKX41
Applications
10