Olympus EHT-421 Buku Petunjuk - Halaman 17

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Center the Condenser and the Light Bulb

After all necessary components are attached to the microscope stand

properly and securely, it is essential to center the condenser and the

light bulb before the microscope is put in operation.

1. First, make sure that all electrical

conne~tions

are done property,

then turn the switch in the microscope base to the ON position.

The lamp will light up. By raising the voltage progressively, you can

ascertain that the bulb is on.

Adjust light intensity to suit your requirements.

2. Swing the auxiliary lens shifting lever on the illuminator base to posi-

tion Observ.

3. Place a specimen on the stage and use the objective lOx to bring the

specimen in focus.

Out of center.

Centered.

Opened fu lIy.

4. Stop down the field iris diaphragm with the field iris diaphragm

control provided on the microscope base. A slightly blurred image

of the field iris diaphragm can now be seen in the field of view.

5. Move the condenser up and down with the condenser height

adjustment knob to focus on the image of the field iris diaphragm.

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Before centration.

After centration.

6. While widening the diameter of the field progressively, use the con-

denser centering knobs to bring the diaphragm image into the center

of the field of view. If the polygonal image of the iris diaphragm

becomes inscribed in the field it means that the field iris diaphragm is

centered. Slightly increase the diameter of the field iris diaphragm

until it is iust outside the field of view.

7. Remove one of the eyepieces from the observation tube, and look into

the eyepiece tube so that the filament image of the bulb at the rear

focal plane of the objective can easily be seen.

8. Center the filament image with the two coaxial centering knobs on the

lamp socket.

.. Before re-insertion of the eyepiece into the observation tube, move

your head to the right or left to ascertain that the filament image is in

center at the rear focal plane of the objective while looking into the

eyepiece tube.

I f there is illumination irregularity seen in the field of view after centra.

tion of the bulb filament, loosen the clamping screw for positioning the lamp

socke~

and move the lamp sod<et back and forth slowly and clamp with the

clamping screw when even illumination is obtained.