Olympus Fluoview-1000 Panduan Pengguna - Halaman 2

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1. Add a Scale Bar to Your File

V.M. Bloedel Hearing Research Center, Core for Communication Research

Center on Human Development and Disability, Digital Microscopy Center

Scan mode and sequential capture

Using Channel Groups, Figure 9A.

Adjusting the channels with sequential imaging

Adjusting the channels with simultaneous imaging

Collecting a Z-Series.

Determine the depth of the optical volume.

Which way does the focus move?

Settings for z-series collection.

What is axial resolution?

Vertical Sections.

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Files: Saving and Transferring

Saving Files

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The Olympus "OIB" format.

The Olympus "OIF" format.

Where are Your Files on the Confocal?

Export file types.

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Add a scale bar to your file

Opening your Files.

Olympus free file viewer

Offline Fluoview workstation

Importing Olympus datasets into ImageJ.

Loci-tools Plugin for ImageJ.

Import Channel Order Macro.

Image 5D plugin.

File Import from the OIF.Files directory.

File Transfer.

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File transfer to Mac Pro 'Fluoview', confocal file server.

Saving files directly to Mac Pro 'Fluoview'.

USB and Firewire Ports on the Mac Pro.

Recording to CD-ROM and DVD-ROM on the Mac Pro.

Networked file transfer to remote servers from the Fluoview Mac Pro

Using FTP to Windows computers

Remote access to the Mac Pro from your laboratory.

Optimizing Image Collection

Qualities of an Optimal Confocal Image.

Overview of image capture properties

Overview of basic image capture.

Basic settings for confocal microscopy

Labeling Controls.

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Negative controls

Positive controls

The confocal aperture

Optimal Iris Diameter

May 11, 2011

Olympus Fluoview-1000 User's Guide

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