Olympus Fluoview-1000 Panduan Pengguna - Halaman 7

Jelajahi secara online atau unduh pdf Panduan Pengguna untuk Mikroskop Olympus Fluoview-1000. Olympus Fluoview-1000 50 halaman.

V.M. Bloedel Hearing Research Center, Core for Communication Research

Center on Human Development and Disability, Digital Microscopy Center

1.1 Place the sample on the microscope

1. Make sure the objective lens is not extending above the level of the specimen holder;

a. If it is, rotate the focus knob to lower the objective lens (set to Coarse Focus)

2. Press 'ESC' button behind either focus knob to lower the objectives;

3. Move the condenser up, out of your way;

4. Install the appropriate specimen holder in the stage, if needed;

5. Place the slide on the stage with the coverslip downwards;

6. The sample should be centered in the stage opening to avoid bumping the sample holder with the

lens as you move the stage to position the desired field of interest;

7. Press 'ESC' button to return the lens to focus;

8. Lower the condenser back into position;

9. Do not use the keypad on the front of the microscope, it has been disabled;

10. All microscope control must be through the Fluoview software windows, Figures 2, 3;

11. Open the Microscope Controller window, if it is not open: Fluoview

menu>Device>Microscope Controller..., Figure 2.1;

12. Select an objective: Microscope Controller Window>Objective Lens>click on desired lens,

Figures 2.4 and 3.2, see Table 2;

13. Condenser setting: Microscope Controller Window>Condenser sets condenser automatically –

don't touch, Fig. 3.1;

14. Select mirrors (fluorescence filter cubes): Microscope Controller Window>Mirror>click on

desired mirror, Fig. 3.3, see Table 1;

15. Fine focus/coarse focus: press the 'F/C' button below each focus knob, Figure 12.2, to toggle

between focus modes;

16. Lower/raise lens to apply immersion oil: press the 'ESC' button behind each focus knob, Figure

12.3;

17. Center the region of interest in the field of view with the joystick;

1.2 Finding the sample – transmitted light or DIC

1. Center the sample over the stage opening to avoid bumping the stage with the lens;

2. Refer to Microscope Controls, Sections 7.2 and 7.3, for details on using DIC and setting Koehler

illumination;

3. Open the Microscope Controller window, Fluoview menu>Device>Microscope Controller...,

Figure 2.1;

4. All microscope control must be through the Fluoview software windows, Figures 2.1, 2.4;

a. Select lens: Microscope Controller Window>Objective Lens, Figure 3.2, Table 2;

b. Condenser controls are set automatically: Microscope Controller Window>Condenser –

don't touch, Fig. 3.1;

c. DIC condenser settings are automatically selected when transmitted lamp is on:

Microscope Controller Window>Mirror>Select DIC (DICT), Fig. 3.3;

5. Turn on the transmitted light;

May 11, 2011

Olympus Fluoview-1000 User's Guide