Olympus U-RFL-T Petunjuk Manual - Halaman 16

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Olympus U-RFL-T Petunjuk Manual
6

Centering the Mercury Burner

The mercury burner emits light by means of discharge produced when a current is supplied across the poles. If the
positions of the poles are moved for example during replacement of the burner, the light would be improperly incident
to the specimen and the observation image becomes dark. If this happens, it is necessary to adjust the positions of the
poles. This operation is called the centering of mercury burner.
} The mercury burner centering is not required every time before observation, but is recommended after the burner has
been replaced or the observation image is dark.
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Fig. 3
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Fig. 4
Fig. 5
· Operation with BX3-RFAS
} Set the main switch of the power supply unit for lamp housing to " I " (ON)
and wait until the illumination light stops flickering and its brightness is
stabilized.
1. Close the shutter by sliding the shutter knob @ to position marked {.
2. Engage a mirror unit other than the ones for U-excitation (U-FUW, U-FUN,
U-FUNA) in the light path. You may use a fluorescence mirror unit for
U-excitation if you do not have other mirror units but, in this case, you
must always view the images through an anti glare plate.
3. Engage the 10X objective in the light path and place the U-CST centering
target on the stage.
4. While looking into the eyepieces, focus on the cross lines for reflected
light (white surface).
5. While looking into the eyepieces, adjust the stage to bring the center of
the cross lines for reflected light (on the white surface) on the center of
the field of view.
6. Turn the revolving nosepiece to engage the empty position (the objective
cap should be removed) in the light path.
7. Open the shutter by sliding the shutter knob @ to position marked \.
The image by scattered light points will be projected on U-CST.
8. While observing the image on U-CST, center the mercury burner by
following steps below.
Centering procedures for Mercury Burner
(i ) Turn the centering knob 3 (the upper knob) to adjust the image on
U-CST to be the brightest.
(ii) Turn the centering knob | (the lower knob) to adjust the image on
U-CST to be the brightest.
} While adjusting (i) and (ii), the bright part of the image can be out
of the center of U-CST.
(iii) Turn the collector lens focus knob 2 to bring only a part of light to
be remarkably bright. (See Fig. 5).
} In actual, the bright part may not necessarily be displayed in the
upper left area as shown in Figure 5.
(iv) Turn the centering knob 3 and | to adjust the center of the cross
lines of U-CST to be the brightest.
(v) Repeat step (iii) and step (iv) to improve the accuracy for adjust-
ment.
This completes the centering of Mercury Burner.
9. Engage the 10X objective in the light path and while looking into
eyepieces, turn the collector lens focus knob 2 to place to the brightest
position.