Nikon DS-5M Petunjuk Manual - Halaman 19

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When the camera head is mounted on a microscope, adjust the microscope as described below.

(1) Use the Appropriate Filters

When capturing a color image:

Insert the NCB filter into the optical path.

When capturing a black & white image:

Insert the appropriate filter into the optical path, depending on the photographic purpose.

Generally, inserting a green interference filter (GIF) will heighten contrast. Contrast is

enhanced by inserting a filter with a color complementary to the specimen.

Supplement

The method for inserting and removing a filter varies from microscope to

microscope. Refer to your microscope's instruction manual.

A third-party color compensation filter (CC filter) can be inserted into the

illumination optical path of the microscope to compensate for changes in

color balance caused by the length of the exposure time.

When using a phase contrast microscope or an interference microscope (two

luminous fluxes or multiple luminous fluxes), you can enhance contrast

using a green interference filter (GIF) or a monochrome interference filter

(IF).

Some microscopes may require a heat-wave absorption filter.

Terms

What is an NCB filter?

An NCB (neutral color balance) filter is a color-balancing compensation filter used to adjust

color temperature to daylight values in microscopes that use a halogen bulb as a light

source.

What is a complementary color?

For example, green and magenta, red and cyan, and blue and yellow are all pairs of

complementary colors. When complementary colors overlap, their respective hues cancel

each other out.

Chapter 5

Microscope Adjustment

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