Nikon A1+ Prosedur Operasi Standar - Halaman 4

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Confocal Acquisition: Quick Start

Setup your channel requirements

HV (Gain), start at 100 for 405/640 and 25 for 488/561 PMTs

Offset adjustment, leave at 0

Laser Power, start at 2%

Start with a scan size of 512x512 and scan speed of 2.2 µsec.

Select "Normal" and "Ch Series" if using more than one laser

Select 1.2 AU for Pinhole

Start "SCAN"

Adjust detector and lasers settings if needed

10. Change pixel size by manually changing zoom factor in A1+ Scan Area" dialog

(View>Acquisition Controls>A1 Scan Area) or pressing Nyquist for optimal pixel

size for the selected objective and pinhole size.

11. Quickly Capture an image with the current settings. Image must be saved

manually afterwards. Save as .nd2.

*If you need to go back to the eyepieces to check your slides, click "Eye Port",

click it again to return to the confocal!

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