Nikon C1si Panduan Pengguna - Halaman 7
Jelajahi secara online atau unduh pdf Panduan Pengguna untuk Mikroskop Nikon C1si. Nikon C1si 7 halaman. Spectral laser scanning confocal microscope
Halaman CetakThe z stepper motor is not working
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The Make sure that the knob is pulled up on the rear left of the microscope.
If
y ou cannot see anything in the eyes (epi) follow this checklist:
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The arclamp (Intensilight) is turned on.
The shutter above the nosepiece is open.
Number 1-5 is selected on the filter wheel.
The eyepiece/scanhead changer is pushed in.
You are focused on your sample.
The aperture on the Intensilight source is at position 1 (maximal transmitted light).
If you cannot see anything on the computer (confocal), follow this checklist:
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You are focused on your sample.
The filter wheel is set to 6.
The eyepiece/scanhead changer is pulled out.
Both the detector and laser are turned on so they are colored.
The detectors that you want are moved to 6.5 or higher.
The sliders for the Lasers that you want are on.
Your sample is getting photo-bleached too quickly:
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Try using less laser power and more detector gain.
Try focusing using single frames instead of live.
Your image is too noisy:
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Try increasing laser power and decreasing detector gain.
Optimizing to get higher resolution:
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Use a higher NA objective.
Zoom in by clicking on the cube in the top right corner of the screen. It will tell you
when this has been reached when the NON OPTIMAL PIXEL SIZE disappears. This
happens when you get Nyquist sampling of (0.61*emission wavelength)/Numerical
Aperture so that you have 2.7 pixels per diffraction limit.
Troubleshooting
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