Nikon Eclipse E400 Petunjuk Manual - Halaman 11

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Preparations for Microscopy

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Turn on the power switch.

(Flip the switch to the "

" side.) As soon as the power comes on, the switch lights.

Place the NCB11 filter provided on the field lens and adjust the

brightness adjuster to a suitable brightness.

Diopter and Interpupillary Distance Adjustments

These adjustments are performed to adjust the microscope to the person using it. Continued use of the microscope without

properly adjusting diopter and interpupillary distance can cause eye strain. Make sure to adjust the microscope properly whenever

you use it. Diopter adjustment compensates for differences in vision between the left and right eyes.

In addition to making

observation through both eyes easier, this adjustment also reduces the defocusing when changing the objective. Perform diopter

adjustment for both eyepieces, respectively.

3-1

= Turn the diopter adjustment ring on the eyepiece to align

the edge of the diopter adjustment ring with the engraved

line. (This is the diopter adjustment "0" position.)

The diopter adjustment

"O" position

3-2

Turn the coarse and fine focus knobs to focus on the specimen with the 40x objective.

(When it is difficult to focus on

the specimen, first focus using the 4x or 10x objective, and then switch to the 40x objective.)

Since the distance

between the objective tip and the top of the cover glass is indicated as the working distance (WD) on the objective barrel,

refer to that distance when focusing on the specimen.

3-3

Move the 4x or 10x objective into the optical path. Turn

the diopter adjustment ring on the eyepiece, not the coarse

and fine focus knobs, to focus on the specimen.

Do so

while peering through the right eyepiece with your right

eye and the left eyepiece with your left eye.

3-4

Adjust the spread of the binocular part to the distance

between your eyes so that the field of view for each eye

is aligned on one position when viewing with both eyes.