Nikon Eclipse Ti TIFT 1454 Manual - Halaman 6

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Tab: Display:
• Auto scale option
o Enable Auto scale or change custom auto scale
o Full bit-range is saved to file, this is only for visualization
in the software
• Visualize saturated pixels
Tab Acquire:
• Be sure to set the Illumination choice to
"Current shutter"
Tab Special:
• Gain options:
• 10 MHz (EM gain), gain3 3x is standard setting
• Control gain between 0 and 1000
• Use "Frames To Avg" option to average images / or use
longer exposure time
Live image window
• Magnifying glass
• Display image in other LUT
o Grayscale, Color by wavelength
• Histogram of intensities
o Manual scaling can be done with the orange arrows
• Auto scaling options
o Best fit range, 8/12/14/16 bit
o Auto scale on/off
o Scale within region
Martijn de Gruiter
Manual: TIRF Microscope
Erasmus OIC
Ee-1454
6