Nikon N-STORM Panduan Pengoperasian - Halaman 10
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When MPB Communications Inc.'s 647-nm laser is
used
(1)
Start the laser control software GUI-VFL.
(2)
Click the [On] button in the window to turn on
the 647-nm laser.
The value of [SHG temp.] is displayed.
(3)
Follow the procedure below to gradually
increase the output of the 647-nm laser through
GUI-VFL (procedure recommended by the
laser maker).
Set the power to 50 mW, click [Activate], and
then wait until the value of [Power, mW]
becomes about 50 mW.
Then, set the power to 200 mW, click [Activate],
and wait until the value of [Power, mW] reaches
about 200 mW. The value of [Power, mW] may
change by a few percent, but this does not
affect the acquisition of STORM images.
After the completion of this procedure, use
NIS-Elements to adjust the laser power. It is not
necessary to use GUI-VFL for adjustment.
(For details on the procedure for turning off this laser,
see step 1 in "2.4 Terminating the N-STORM
System.")
3
Start NIS-Elements AR.
(1)
Start NIS-Elements AR by double-clicking the
corresponding icon.
(2)
When the camera driver selection dialog is
displayed, select [ANDOR with N-STORM]. (To
change the camera driver after NIS-Elements is
started, select [Select Driver...] from the [Acquire]
menu.)
(3)
Display the [N-STORM] control window and the
[DU-897 Settings] (camera) control window. (Select
[Acquisition Controls] from the [View] menu and then
select the control windows.)
Chapter 2
STORM Microscopy Operation
6
(1)
(2)
(3)
(3)
3-(1)
3-(2)