ATLAS BIOTECNOLOGIES AB20 Manuale di istruzioni - Pagina 8

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One full turn of the calibration key changes the pipette

aspiration volume by the amount given in the table.

• Take out the key and fix the pipetting pushbutton (Fig. 4D).

The pushbutton should be fixed by placing first the knob

on the arbor (Fig. 1A2) and then the pushbutton (Fig. 1A1).

Determine the average aspirated volume. The average

volume should be within the permissible range given in

the table. If the volume exceeds the values stated, the

recalibration procedure should be repeated.

In case of pipetting the liquids with physical properties

considerably different from those of water, follow the

rules given in section 5.

9 - TROUBLESHOOTING

If you notice an improper pipette operation identify the

cause and eliminate the fault. Doing this, follow the

instruction in the sequence provided. Replacement of

elements into new ones may be required only exception-

ally, and should not occur under normal pipette use.

Droplets of liquid remain in the pipette tip.

• The tip is emptied too fast.

Decrease the speed of pressing the pipette push-

button.

• The tip wettability has increased due to extensive use.

Replace the tip with a new one.

Droplets of air appear in the liquid aspirated into the tip.

• The pipette tip immersion is too shallow.

Immerse the tip deeper according to the instruc-

tions.

• The pipette tip is incorrectly pressed onto the pipette

shaft.

Press the pipette firmly.

• The tip is damaged or worn out due to extensive use.

Replace the tip with a new one.

The pipette incorrectly aspirates the liquid or liquid

drops out from the tip.

• The pipette tip is incorrectly pressed onto the pipette

shaft.

Press the pipette tip firmly.

• The shaft nut is loose (Fig. 3F).

Tighten the shaft nut.

• The sealing surface of the shaft is cracked or scored.

Remove the tip ejector. Unscrew the shaft nut,

inspect the shaft and the piston assembly. Replace

the damaged parts (see section 12). When reassem-

bling the pipette, the nut should be hand tightened.

In the models AB10 and AB20, the damage of the shaft

may also cause a damage of the piston assembly.

Replace the damaged parts (see section 12).

When reassembling the pipette, the nut should be

hand tightened.

• Damage of the piston or seal due to prolon ged use with

the aggressive liquids.

Disassemble the pipette as described above.

Replace the piston, seal and O-ring (see section 12).

Rinse the inside of the shaft in distilled water and dry.

Lubricate the seal and O-ring with the lub ri cant.

The replacement of the piston requires conduc ting

of calibration procedure.

Note: The parts of AB10 pipette should be lubricat-

ed evenly with the minimum amount of lubricant.

• The pipette is reassembled improperly.

Disassemble the pipette and reassemble it, obser -

ving the proper sequence of steps (Fig. 3).

• No lubricant on the sealing elements.

Remove the tip ejector. Unscrew the shaft nut,

remove the shaft, piston assembly, seal and O-ring.

Rinse the removed parts in distilled water and dry

thoroughly. Lightly lubricate the inside surfaces of

the seal and the O-ring with the lubricant. Reas -

semble the pipette in the reverse order.

• Contamination of the inside of the pipette caused by

extensive aspiration of chemically aggre ssive liquids or

because liquid got inside the pipette.

Remove the tip ejector. Unscrew the nut, remove

the shaft, piston assembly, seal and O-ring. Rinse

the removed parts with distilled water and dry thor-

oughly. Lightly lubricate the inside surfaces of the

seal and the O-ring with the lubricant. Reassemble

the pipette in the reverse order.

Note: All parts may be autoclaved at a temperature of

121°C for 20 minutes at pressure 1 bar.

The handle and the outside of the pipette may be

cleaned using a cloth damped in isopropanol.

ENGLISH