Drummond Nanoject II Manuale di istruzioni - Pagina 5

Sfoglia online o scarica il pdf Manuale di istruzioni per Unità di controllo Drummond Nanoject II. Drummond Nanoject II 12. Digital control box

Plastic sleeve (spacer) – Placed between

O-rings with indentation toward glass

Aluminum Barrel

Sealing O-ring (small hole)

Fig. 1

7) Filling the Micropipette

First, select your fill speed by pressing the [SETUP] icon and choosing 23 or 46 nL/sec.

(NOTE: EMPLY SPEED IS PRESET TO 92 nL/SEC ONLY)

Return to Main Menu by pressing the [EXIT] icon.

Then enter manual mode by pressing the [MANUAL] icon.

Once the micropipette is secured to the collet, press the [EMPTY] icon. This will drive

the wire plunger out forcing oil to the tip of the pipette and any excess oil will be

expelled. Extend the plunger until an audible beep is heard. The plunger is now fully

extended (approx. 27 mm from the end of the collet).

Now place the tip of the pipette into your sample and press the [FILL] icon. The

plunger will retract drawing with it the sample. The micropipette when fully filled, will

contain approximately 4.2 µl of sample. At any time you can stop filling by pressing the

red [STOP] icon. Continued filling can be accomplished by pressing the [FILL] icon

again. The plunger is fully retracted to its preset "home" position when you hear an

audible beep. Viscous samples may require you to fill in small steps allowing the

sample to equilibrate in the tip before continued filling. Do not allow air bubbles to form

in the micropipette. These bubbles can cause inaccurate injection volumes.

NOTE: The plunger will continue to extend or retract until you press [STOP], or fully

extended or retracted position is reached.

8) Injecting Sample

To inject the sample, return to the Main Menu by pressing the [EXIT] icon. Press the

[SETUP] icon then choose your Speed (nL/sec), either 23 or 46 nL/sec. Then press

the [EXIT] icon. Select inject mode by pressing the [INJECT] icon. Set the desired

injection "Volume (nL)" from the displayed volumes. Press the [INJECT] icon to inject

your sample.

NOTE: Multiple injections can be performed by simply pressing the [INJECT] icon

again and again.

Plunger

Collet

O-ring with large hole

Glass