Olympus BX-RLA2 Manuale di istruzioni - Pagina 12

Sfoglia online o scarica il pdf Manuale di istruzioni per Microscope Olympus BX-RLA2. Olympus BX-RLA2 32. Reflected light brightfield/darkfield illuminator

Stampa la pagina

Fig. 3

70%

30%

Aperture iris diaphragm image

Centering the Aperture Iris Diaphragm (AS)

1. Slide the mirror selector lever @ to "BF".

2. Engage the 10X objective by rotating the revolving nosepiece and bring

the specimen in approximate focus.

3. Remove the eyepiece, look into the eyepiece sleeve and pull the AS knob

² so that the aperture is about 70%.

4. If the center of the iris diaphragm is deviated, center it by rotating the two

AS centering screws ³ using the Allen screwdriver.

Using the Aperture Iris Diaphragm

{In reflected light brightfield observation, optimum observation is gener-

ally possible by setting the aperture to between 70% and 80% of the

aperture number of the objective.

{In reflected right darkfield observation, the aperture must be fully opened

by pushing in the AS knob ².

}With some specimens, an image with high contrast and little flare may

sometimes obtained when the aperture is slightly closed. It is therefore

recommended to also try a slightly closed aperture.

(Fig. 3)