Olympus BX60 Manuale di istruzioni - Pagina 32

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BX60

Aperture Iris Diaphragm

53> I

illumination system. Matching tho numerical aperture ol the illumination

system with that of the objective provides better image resolution

and contrast, and also increases the depth of focus.

• Since the contrast of microscope specimens is ordinarily low. setting

the condenser aperture iris diaphragm to 70*0% o! the NA of the

objective in use is usually recommended. When necessary, adjust the

ratio by removing the eyepiece and looking into the eyepiece sleeve

while adjusting the aperture iris diaphragm ring (J) until the image

shown in Fig. S3 is seen.

O Using the Numerical Aperture Seals

. (fig. 54)

Example:

With the Plan 40X (NA 0.651. set the scale to 0.65 X 0.8 - 0.5.

the

Compatibility of Objectives and Condernari

•I W'w usir* ir» U-SC siwnfrou a(±>omat condenw toc^^

and um Ota field ins itsphngm m tha bssa n aperture diashrscm.

•2 AllhoushslishttyinadoQuataNA rosults in a wnwrvtat daitcr feld of vi«w vwth a I00X objacUw. tlw o(OT*™

O To otaoi banar JBumrowm. usa of tha UHJLC a (ocemmendad m pnmomoography whan using tha 2X or 4X cbfiar*.