Olympus FV1200 MPE Manuale d'uso - Pagina 3

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Olympus FV1200 User guide

C. Outline of Acquisition Setting / Image Acquisition/ Image Viewer Windows

Time Scan: set up the time lapse series condition (interval and number of image acquisition.

Before taking images, let's get familiar with buttons and sliders on the

Acquisition Setting Window (such as scan mode, scan speed, image

size etc).

Scan mode: the default is unidirectional

Scan Speed: The higher value, the slower, the better image. Around

12.5~20 us/pixel (microsecond per pixel) is a good speed.

Image size (number of x,y pixel): 512x512 pixel is the default.

Area: the image field can be optically zoomed in and rotated.

To rotate the viewing area, click on the red dot and drag clock- or

counter-clockwise (or click

slider (click

to return to 1x). With zoomed view, you can select the

scanning area by moving the blue-lined box around.

Laser power control: Checking the box will activate the laser. The

power can be adjusted by pointing the mouse pointer to the slider

area and clicking (large change), clicking the arrow heads (small

change), or rolling the mouse wheel on the slider area.

Lambda Scan: for spectral imaging.

Microscope: Objective selection is displayed. Also selecting the

objective on the dropdown list will automatically change the objective.

Z position setting: Clicking the large arrow heads

objective up or down to focus onto the sample. The extent of

movement is determined by the Step size. Clicking small arrow heads

will move objective in half the Step size.

Start Set and End Set determine the z series range.

). Zoom the imaging area with the

June 23, 2015

will move the