Olympus FV3000 Manuale operativo - Pagina 30

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（acquiring by a single channels）

⑤

⑥

Figure(A)

①

②

⑧

Standard

・Bandwidth：

15μm is recommended. If the live image

is dark, widen the Bandwidth.

・Step Size：

5nm is recommended. The narrower the

Step Size, the more accurate the analysis.

Lambda series

⑦

⑤

④

*Before starting the following procedure,

make adjustments for imaging.

(refer to page 4-8.)

Changing to Lambda mode

① Select "Lambda" in [Mode] in [PMT

setting] Tool Window.

Select one channel to be used

② Press the

[Dye & Detector Select] dialog box

appears. Press the

the Assigned Detector.

③ Select the [Channel] tab in [Dye &

Detector Select] dialog box. Select the

"High Sensitive Detector CH1" in

[Detector List], and press

Press the

button to close the [Dye &

Detector Select] dialog box.

④ Tick the checkbox of the wavelength of

the excitation laser to be used.

Setting DM and SDM

⑤ Select [Lightpath] in [Tool Window]

menu. [Lightpath] Tool Window appears.

⑥ Select [LSMScanner] tab in [Lightpath]

Tool Window. Press the DM button to

display the dichroic mirror list. Select

"DM" which reflects the wavelength of

the excitation laser selected in ④.

⑦ Check the light path whether the light

path is set properly. See the figure(A).

（SDM→MIRROR→MIRROR→HSD1)

Setting "Band width" and "Step Size"

⑧ In [PMT Setting] Tool Window, set the

width of the wavelength for which the

spectral image acquisition is performed

per each step in [Band width], and set

the interval between the wavelength to

start acquisition in the next step in [Step

size].

button. The

button to reset

button.