Olympus IX70 Manuale operativo - Pagina 17

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gives a live stack display. This menu closes after you use it.

The last two items are not general user items. Please don't alter settings. (setting camera to 8-bit

mode can speed data capture, but be kind and restore 12-bit mode IF you use this option).

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Acquire submenus

You should open the Acquire/Acquire menu each session

to access camera controls

You can use the AcquireZseries menu to execute Z stack

collection. You will need to set limits using the Devices/Focus

menu first. This menu closes after you use it.

AcquireMultipleWavelengths permits automated

collection of up to 6 data channels. This menu stays open.

StreamAcquisition collects data as fast as the system can

run. Data is not shown live, but streamed to memory. You can

review it as soon as a run stops.

AcquireTimelapse permits slower image capture and

Devices submenus

Most submenus are not meant for general user modification.

An exception is the FOCUS menu. You can use it to alter the

sensitivity of the focus knob on the microscope. Choosing an

objective does input a preset sensitivity, but if you find the action is

too coarse or fine, select another value from the pulldown list under

the SPECIAL tab. You can use this menu to set the limits for a z-

series collection via the Acquire/AcquireZseries menu. The

Apps/AcquireMultiDimensionalData routine has its own built-in tab

for setting Z limits.

The Configure menus define the microscope settings and

should not be altered. If you need a special setting, ask the staff to

create/modify a custom setting for you.

Display submenus

Most functions to alter the appearance of images are located here. The

AdjustDigitalContrast and ScaleImage menus control the appearance on the

screen (as opposed to data in the image).

See/use the Taskbar shortcut buttons for common operations such as setting

scaling, color, etc.

"Contrast Shortcuts" HERE is archaic and most operations do NOT work for

12-bit image data

Graphics has annotation tools (see below). USE THESE for scalebars and

other calibrations (resist using arrows and text—they are poor quality)

IntensityProfile creates 3D histogram plots of intensity as height

Use OverlayImages to combine dyes and DIC images

ColorCombine is an alternate tool for overlays, esp. good for making RGB

images for true color captures.

ColorAlign corrects chromatic aberrations