Accuris SmartDoc 2.0 Manuale di istruzioni - Pagina 6
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Stampa la pagina7. GEL IMAGING WITH THE SMART DOC
Electrophoresis gels should be prepared and stained according to the instructions
included with the nucleic acid stain. Use the proper type of transilluminator for
the stain used. Common stains that work well with the SmartDoc enclosure and
filters include:
SmartGlow™ PS (Pre Stain), SmartGlow™ LD (Loading Dye), SYBR® Green I and II,
SYBR® Safe, SYBR® Gold, Gel Star, Gel Green, and Green Glo™, Ethidium Bromide,
Gel Red.
1. The UV or blue light transilluminator should be set up on a level and stable
lab bench or table.
2. Carefully place a stained electrophoresis gel onto the transilluminator
viewing surface.
3. Note: The gel to be imaged should be 15x15cm or smaller. Cut the gel to a
smaller size if required.
4. Note: If the transilluminator being used has a viewing surface larger than
19x19cm, please use E5000-MAT UV Blocking Mat. This will block any UV
or blue light from coming around the edges of the SmartDoc enclosure
base.
5. Place the SmartDoc imaging enclosure onto the transilluminator.
6. Insert the appropriate filter into place on the top platform. The side with
the Accuris logo should be facing up. See Section 6 for information on
filters.
7. Place a smartphone face down onto the top platform, and align the camera
lens with the filter. NOTE: Some protective phone cases can prevent the
camera lens from aligning and connecting to the photo filter. Remove the
phone case if necessary.
8. Turn on the power of the transilluminator.
9. Select the Camera Mode on your Smart Phone and turn off the flash
setting. When properly positioned, the gel will be seen in the device's
display screen. Focus as required.
10. If required, remove or insert the extender piece to maximize the image size
of the gel in the display. NOTE: Always turn of the power of the
transilluminator when adjusting the SmartDoc enclosure.
11. A zoom function on the camera phone can be used to enlarge the gel
image in the display, but this can decrease the resolution of the image.
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