Nikon TIRF Manuale di istruzioni - Pagina 17

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TIRF cassette holder

Filter blocks that incorporate dichroic mirrors are attached. This holder reflects the laser beam from

the TIRF attachment to guide it to the objective. Up to six filter blocks can be attached and replaced

as necessary.

To protect your eyes and skin from exposure to the laser, do not detach the TIRF cassette holder

from the microscope.

Left side

(1) Excitation method switching ring

Rotate this ring to switch between filter blocks.

(2) Shutter lever

Move the lever clockwise to move the shutter into the optical path, thus interrupting the laser

beam.

In general, leave this lever in the "O" (open) position and manipulate the shutter control unit to

interrupt the laser beam with the motorized shutter.

(3) Filter block mount-cover

Make sure that all lasers are off when replacing filter blocks.

Remove this cover to replace filter blocks.

Replacing filter blocks:

(To clarify the description of the procedure, the diagram of the epi-fl cassette holder that does

not have the upper cassette holder is shown.

See next page.

I Name and Function of Each Part

TIRF cassette holder

(1) Excitation

method

switching

ring

(3) Filter block

TIRF cassette

mount-cover

holder

Illustration of the TIRF cassette holder

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Diagram of the epi-fl cassette holder

(2) Shutter lever

Right side