Nikon L-ke Manuale di istruzioni - Pagina 5

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OBJECTIVES, E Y E P I E C E S , CONDENSERS

1. Objectives

OBJECTIVES for medical and biological use

Type

Individual

Magnification

Numerical

Focal

Aperture

Length

Working

Distance

Remarks

Achromatic Dry

Oil-immersion

4X "

lOX

20X

40X

S40X

NC40X

lOOX

SIOOX

lOOX

0.10

0.25

0.40

0.65

1.25

28.3mm

14.8mm

7.5mm

4.3mm

1.8mm

9 . 5 0 l i m

7.10mm

5.70mm

0.54mm

0.52mm

0.16mm

Spring-loaded

No cover glass type

Spring-loaded

With iris diaphragm for dark-field

Plan Achromatic Dry

Oil-immersion

Plan 1.2X

lOX

40X

NC40X

lOOX

0.03

0.05

0.08

0.10

0.25

0.65

1.30

35.8mm

42.3mm

37.7mm

29.5mm

15.6mm

4.0mm

4.1mm

1.6mm

29.7mm

35.6mm

28.6 mm

18.2mm

7.0mm

0.24mm

0.32mm

0.12mm

Spring-loaded

Spring-loaded, no cover glass type

Spring-loaded

Plan Apochromatic

Oil-immersion

Apochromatic Dry

Oil-immersion

lOOX

40X

lOOX

0.80

„ _ 1 - 4 0

1.6mm

4.3mm

L 6 m m

0.12mm

0.19 m m '

0.10mm

Spring-loaded

Long-Working Distance Type

Achromatic Dry

LWD40X

4.0mm

2.0 mm

For tissue culture observation

Phase-Contrast

Achromatic Dry

Oil-immersion

Long-Working Distance

Achromatic Dry

DLLIOX

B-MIOX

DLL20X

B-M20X

DLL40X

D-M40X

B-M40X

DLLIOOX

D-MIOOX

B-MIOOX

D-M40X

0.30

0.40

0.65

1.25

0.60

15.9mm

8.2mm

4.4mm

4 . 4 m m

4.4mm

1.8mm

4.0mm

6.4mm

4.5mm

0.54mm

0.16mm

2.0mm

Dark Contrast

Bright Contrast

Dark Contrast

Bright Contrast

Dark Contrast, Spring-loaded

Dark Contrast

Bright Contrast

Dark Contrast

Bright Contrast

Dark Contrast

(For tissue culture work)

OBJECTIVES for metallurgical use

Metallurgy

Achromatic Dry

M5X

MlOX

M20X

M40X

MIOOX

0.10

0.25

0.40

0.65

1.25

25.0mm

14.8mm

7.5mm

4.3mm

1.8mm

15mm

7.1 mm

5.7mm

0.52mm

0.16mm

Plan Achromatic Dry

Plan

MlOX

Plan M40X

Plan MIOOX

0.25

0.65

1.30

15.6mm

4.1 mm

1.6mm

7.0mm

0.32mm

0.12mm

Spring-loaded

2. Eyepieces

Type

Individual

Magnification

Focal

Length

Field

Number

Remarks

High eyepoint, compensating

HK5X

50mm

21.0

With adjustable eyepiece collar

High eyepoint, compensating,

wide-field

HKWIOX

25mm

18.0

With adjustable eyepiece collar

High eyepoint, compensating,

wide-field

HKW15X

16.7mm

14.0

With adjustable eyepiece collar

Compensating, wide-field

WFIOX

25 mm

18.0

High-power purpose

Compensating

K20X

12.5mm

8.0

General purpose

Huygenian

H5X

HlOX

H15X

50mm

25mm

^17nim

21.0

12.0

8.0

General purpose

Diopter adjustable, high eye-

point, compensating, wide-field

DHKWIOX

25mm

18.0

Supplied together with

" U " eyepiece tube

With 5X, lOX, 15X picture frames plus cross-

lines for framing and focusinsf

3. Combinations of Objectives and Eyepieces

Total magnifying power obtained by the combination is the product of

individual objective power multiplied by individual eyepiece power.

selection of the combination will be decided so as to get the highest

resolution of the image (resolving power), the largest extent of object

area (real field) which can be observed without moving the stage or

slide, or the greatest thickness of object (depth of focus) which can

be distinctly seen without raising or lowering the microscope stage,

depending upon the purpose of using the microscope. Shown below

are the results compiled from the different combinations of objectives

and eyepieces:

Objective

Eyepiece

Total

Magnifying

Power

Working

Distance

Resolution or

Minimum

Resolved

Distance

Real Field

of View

Depth of

Focus

Objective

Eyepiece

Total

Magnifying

Power

(mm)

in object

(^)

in image

(mm)

(^)

lOX

15X

20X

40X

60X

9.5

2.7-5.5

0.05-0.11

0.11-0.22

0.16-0.32

5.25

4.5

3.5

100

lOX

15X

50X

lOOX

150X

7.1

1.1-2.2

0.05-0.11

0.11-0.22

0.17-0.33

2.1

1.8

1.4

20X

lOX

15X

lOOX

200X

300X

5.7

0.69-1.38

0.07-0.14

0.14-0.28

0.21-0.42

1.05

0.9

0.7

40X

lOX

15X

200X

400X

600X

0.54

0.42-0.84

0.08-0.17

0.17-0.34

0.25-0.50

0.52

0.45

0.35

1.8

1.2

1.0

lOOX

lOX

15X

500X

lOOOX

1500X

0.16

0.22-0.44

0.11-0.22

0.22-0.44

0.33-0.66

0.21

0.18

0.14

0.6

0.44

0.38

• The working distance is the clearance between the upper surface of the

cover glass and the lowest edge of the objective critically focused. Note

that, as shown in the above table, the working distance becomes very

small for high power objectives.

• The resolution of minimum resolved distance (the limit of resolving power)

is the minimum distance between object points discernible as separate

under the microscope illuminated by the light of wave length

5 5 0 m y u .

The shorter the light wave length, the higher the resolving power, that

is, the small resolving distance results. In the table, the smaller values

indicate the resolution obtained by oblique and the larger values by

central illumination, (see "Illumination" on page 1 1 )

# T h e minimum resolved distance in the image is the value in the object

multiplied by the total magnification of the microscope. If the resolving

power of the microscope is important, choose the eyepiece by the use

of which the image resolution comes within that of the naked eye 0 . 1 5

—0.3mm (when the object is seen from the distance 25mm); generally

accepted criterion on the upper limit of the total magnification

of a

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