Clontech PT3139-1 Manuale d'uso - Pagina 8

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CLONTECH Laboratories, Inc.

III. Additional Materials Required

The following reagents are required but not supplied.

• Mineral oil (We recommend Sigma

• 0.5-ml PCR reaction tubes (We recommend Perkin-Elmer GeneAmp

0.5-ml reaction tubes [Cat. #N801-0737].)

• Thermal cycler (Perkin-Elmer DNA Thermal Cycler 480, 9600, or equivalent)

• Dedicated pipettors (1–2-µl, 1–10-µl, 1–20-µl, 20–200-µl, 200–1000-µl)

• PCR pipette tips suitable for the above pipettors and equipped with

aerosol-barrier filters. Do not autoclave pipette tips.

• DNA size markers (See Section IV.D)

• 5X Stop/loading buffer (Sambrook et al. [1989] provides several

recipes.)

page

Protocol # PT3139-1

Version # PR76834

Cat. #M-3516.)

Technical Support TEL:415-424-8222 or 800-662-CLON

FAX: 415-424-1064 or 800-424-1350