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*Error messages warn you of compensation values that are >100%. This can indicate one of two

things, the voltage settings used are such that one of your dyes is brighter in an off-channel than it is

in the channel selected and a channel receiving less total photons has been over amplified, or that the

color combination at the settings you have chosen is not optimal. If you are still having problems, it

13. Return to your original Global Worksheet by clicking on the Global Worksheet icon in the Worksheet

Window.

Running Samples

1. Create a FSC-A by SSC-A plot on the global worksheet and create a polygon gate to select your cells.

2. Create a population hierarchy by right-clicking on any dot plot and

selecting "Show Population Hierarchy". Create other dot plots or

histograms as needed for your experiment.

3. Make sure the machine is in RUN.

Load your first sample tube onto the instrument and click "ACQUIRE

DATA".

5. Adjust your FCS and SSC voltages if needed.

6. Select the number of events to record in the Stopping Gate settings on the Acquisition Dashboard and

click "RECORD DATA".

7. Save your analysis template for future use. Click on the analysis global worksheet, right click on

analysis, export template, rename, and export to general.

may indicate that you do not have a good color combination.*