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BD FACSAria スタートアップ、QC、シャットダウン手順
6. Post Sort Cleanup and Shutdown
1
Clean the sorter after appointment is complete and check to see if fluidics and/or
1
cytometer shutdown is necessary.
Before 5pm (business weekdays only):
Clean with 2 minutes CLEAN (bleach) at flow rate 11, 2 minutes RINSE (detergent) at flow rate 11,
followed by 2 minutes H2O at flow rate 11 and leave instrument on.
After 5pm (or any time on weekends and holidays):
If someone is booked after your appointment:
Clean with 2 minutes CLEAN (bleach) at flow rate 11, 2 minutes RINSE (detergent) at flow rate 11,
followed by 2 minutes H2O at flow rate 11 and leave instrument on.
If no one is booked after your appointment the instrument must be shutdown:
Aria-2/4: Perform Fluidics Shutdown followed by an instrument shutdown (see below 2-4).
Aria-7: Saturday through Thursday: turn stream off, place closed loop nozzle and perform a clean
flow cell with water followed by instrument shutdown (see below 4); Friday: perform Fluidics
Shutdown followed by an instrument shutdown (see below 2-4)
1
Change connections between Sheath Fluid tank and EtOH Fluid tank to prepare for
2
Fluidic Shutdown.
EtOH tank connected
3
1
1
Shutdown fluidics.
3
Go to Cytometer  Fluidics Shutdown. Follow on screen prompts.
Note: When prompted to load a tube of cleaning solution, load a tube of 70% EtOH.
1
Turn off cytometer.
4
Turn off the main power switch followed by the laser main switch.
1
Turn off AMS or BSC along with the chiller.
5
Note: Please remember to log out of Diva at the end of your appointment. It is necessary for
accurate tracking of usage and billing.
Sheath tank disconnected
2
Fluidics setup for EtOH shutdown.
1. Fluidics line hooked up to
1
designated EtOH filter.
2. Pressure line hooked up to
2
male connection on EtOH tank.
3. Release valve down, allowing
3
pressurization of EtOH tank.
*To Refill tank when empty,
disconnect pressure line and open
release valve to depressurize EtOH
tank and fill it with 70% EtOH.