- ページ 7
計測機器 Beckman Coulter Vi-Cell XRのPDF ユーザーマニュアルをオンラインで閲覧またはダウンロードできます。Beckman Coulter Vi-Cell XR 9 ページ。 Cell viability analyzer
ページを印刷する- 1. Getting Started with the VI-Cell
- 2. System Specs
- 3. The Software
- 4. Running a Sample
- 5. Camera Image
- 6. Autosampler Queue
- 7. Sample Prep
- 8. Analyzing the Sample Run
- 9. Saving Data
- 10. General System Information
- 11. VI-Cellxr Cell Image Analysis Scheme
- 12. Creating and Modifying VI-Cell Cell Types
- 13. To Access the Cell Types
- 14. Sample Prep Options
- 15. Image Analysis Parameters
- 16. Cmcresson
CMCresson
Creating and Modifying Vi-Cell Cell Types
When you begin using the instrument, it is best to select the Default Cell Type
to analyze your sample. Then, you can adjust these parameters to fit the
specific cell line you are measuring and create a unique cell type. These cell
types will show up in everyone's user account and there should be only one cell
type for each cell line that we use in the lab. Please talk with other lab
members to set the parameters for a given cell line so that everyone can use
the same cell type for a given cell line.
For additional details, please see the Vi-Cell User Manual or the cell type power
point tutorial, which is saved on the desktop of the Vi-Cell computer with the
extension pps.
To access the cell types:
1. choose File from toolbar then click on Cell Type and you will be given
the option to add, modify, or delete a cell type.
2. choose File from toolbar then click on Image-Reanalyze and you will be
given the option to add, modify, or delete a cell type.
3. choose Instrument-Reanalyze from toolbar then you will be given the
option to add, modify, or delete a cell type.
Sample Prep Options
Parameter
Aspiration
Cycles
Trypan Blue
Mixing
Image Analysis Parameters
The ranges given below are the normal recommended ranges for each
parameter and default value refers to the parameter value in the Default Cell
Type. The default values are a good starting place and you should tweak
individual parameters to determine their effects on the output values.
For the cell brightness parameter, you will need to observe the image in binary
mode to see the difference between parameter values. Right click on the
image, then turn off annotation and then choose Binary Mode. Return to
normal view for all other parameters.
Range
Default
1 to 7
3
1 to 7
3
15-March-06
Details
# of times sample is mixed in
syringe; set according to cell
sensitivity & clumpiness
# of times sample and trypan blue
are mixed in syringe